Ascorbic acid Ascorbic acid Ascorbic acid Select Applications HPLC Method Development. Effect of Mobile Phase Composition on Retention and Separation of Active Ingredients of Cold and Flu Drug Composition Quantitative UV HPLC Analysis of Vitamin C in Juices on Amaze HA Column HPLC Separation of Isomers of Ascorbic Acid and Ascorbic Acid-2-Phosphate in HILIC/Mixed-Mode on Amaze HD Column HPLC Separation of Ascorbic Acid and Ascorbic Acid-2-Phosphate in Reversed-Phase and Anion-Exchange Modes on Amaze HA HPLC Separation of Isomers of Ascorbic Acid in Reversed-Phase and Anion-Exchange Modes on Coresep SB Column HPLC Separation of Isomers of Ascorbic Acid in Reversed-Phase and Anion-Exchange Modes on Amaze HA Column HPLC Separation of Isomers of Ascorbic Acid in HILIC and Anion-Exclusion Modes on Amaze HD Column HPLC Separation of Ascorbic Acid and Ascorbic Acid-2-Phosphate in HILIC and Anion-Exclusion Modes on Amaze HD Column HPLC Separation of 15 Organic Acids on Amaze HA Mixed-Mode Column with a Triple Gradient HPLC Analysis of Ascorbic, Citric and Ethylenediaminetetraacetic acids on Heritage MA Mixed-Mode Column HPLC Analysis of 10 Water-Soluble Vitamins in Reversed-Phase, Cation- and Anion-Exchange Modes on Amaze QR Tri-Modal Column HPLC Analysis of 10 Water-Soluble Vitamins in HILIC, Cation- and Anion-Exchange Modes on Amaze TH Tri-Modal Column HPLC Analysis of Ascorbic and Citric Acids in HILIC/Anion-Exclusion Modes on Amaze HD Column HPLC Analysis of Ascorbic and Citric Acids on Amaze TH Mixed-Mode Column HPLC Analysis of Ascorbic, Methylmalonic and Succinic Acids on Heritage N Column HPLC Analysis of Ascorbic and Dehydroascorbic acids on Coresep SB Column in reversed-Phase Anion-Exchange Modes HPLC Method Development. Effect of Mobile Phase Composition on Retention and Separation of Active Ingredients of Cold and Flu Drug Composition Coresep SB PURCHASE LEARN ABOUT Coresep SB Conditions of Experiment Column: Coresep SB Separation Modes: reversed-phase, anion-exchange, cation-exclusion Column Dimenstions: 4.6x150 mm, 2.7 um, 90A Mobile Phase: ACN/water/ammonium phosphate Detection: 225 nm Sample: 0.1-0.2 mg/ml Injection: 2 uL Flow rate: 1 ml/min Analytes Class of compounds: Aromatic acid, Aromatic compound, Drug, Glycoside, Pyridine, Sugar, Supplement, Vitamin Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar Compounds: Acetaminophen, Ascorbic acid, Loratadine, Rutoside Quantitative UV HPLC Analysis of Vitamin C in Juices on Amaze HA Column Amaze HA PURCHASE LEARN ABOUT Amaze HA Conditions of Experiment Column: Amaze HA Separation Modes: HILIC, anion-exchange Column Dimenstions: 3.0x100 mm, 3 um, 100A Mobile Phase: 60% ACN with 10 mM AmAc pH 4 Detection: 275 nm Sample: 0.02-0.5 mg/ml Injection: 2 uL Flow rate: 0.6 ml/min Analytes Class of compounds: Aromatic acid, Supplement, Vitamin Nature of compounds: Acidic, Hydrophilic, Polar Compounds: Ascorbic acid HPLC Separation of Isomers of Ascorbic Acid and Ascorbic Acid-2-Phosphate in HILIC/Mixed-Mode on Amaze HD Column Amaze HA PURCHASE LEARN ABOUT Amaze HA Conditions of Experiment Column: Amaze HA Separation Modes: hydrophilic acidic Column Dimenstions: 3.0x100 mm, 3 um, 100A Mobile Phase: from 85% ACN with 30 mM Ammonium Acetate pH 4to 50% ACN with 10 mM Ammonium Acetate pH 4 in 10 min Detection: 280 nm Sample: 0.2 mg/ml Injection: 2 uL Flow rate: 0.6 ml/min Analytes Class of compounds: Aromatic acid, Metabolite, Organic acid, Supplement, Vitamin Nature of compounds: Acidic, Hydrophilic, Polar Compounds: Isoascorbic acid, Ascorbic acid, Ascorbic acid 2-phosphate HPLC Separation of Ascorbic Acid and Ascorbic Acid-2-Phosphate in Reversed-Phase and Anion-Exchange Modes on Amaze HA Amaze HA PURCHASE LEARN ABOUT Amaze HA Conditions of Experiment Column: Amaze HA Separation Modes: hydrophilic acidic Column Dimenstions: 3.0x100 mm, 3 um, 100A Mobile Phase: from 10% ACN with 10 mM Ammonium phosphate pH 4to 20% ACN with 120 mM Ammonium phosphate pH 4 in 10 min Detection: 280 nm Sample: 0.2 mg/ml Injection: 2 uL Flow rate: 0.6 ml/min Analytes Class of compounds: Aromatic acid, Metabolite, Organic acid, Supplement, Vitamin Nature of compounds: Acidic, Hydrophilic, Polar Compounds: Ascorbic acid, Ascorbic acid 2-phosphate HPLC Separation of Isomers of Ascorbic Acid in Reversed-Phase and Anion-Exchange Modes on Coresep SB Column Coresep SB PURCHASE LEARN ABOUT Coresep SB Conditions of Experiment Column: Coresep SB Separation Modes: reversed-phase and anion-exchange Column Dimenstions: 4.6x150 mm, 2.7 um , 100A Mobile Phase: 20% ACN with 50 mM Ammonium phosphate pH 4 Detection: 280 nm Sample: 0.2 mg/ml Injection: 2 uL Flow rate: 1 ml/min Analytes Class of compounds: Aromatic acid, Organic acid, Supplement, Vitamin Nature of compounds: Acidic, Hydrophilic, Polar Compounds: Ascorbic acid, Isoascorbic acid HPLC Separation of Isomers of Ascorbic Acid in Reversed-Phase and Anion-Exchange Modes on Amaze HA Column Amaze HA PURCHASE LEARN ABOUT Amaze HA Conditions of Experiment Column: Amaze HA Separation Modes: reversed-phase and anion-exchange Column Dimenstions: 3.0x100 mm, 3 um, 100A Mobile Phase: 2% ACN with 5 mM Ammonium phosphate pH 4 Detection: 280 nm Sample: 0.2 mg/ml Injection: 2 uL Flow rate: 0.6 ml/min Analytes Class of compounds: Aromatic acid, Organic acid, Supplement, Vitamin Nature of compounds: Acidic, Hydrophilic, Polar Compounds: Isoascorbic acid, Ascorbic acid HPLC Separation of Isomers of Ascorbic Acid in HILIC and Anion-Exclusion Modes on Amaze HD Column Amaze HD PURCHASE LEARN ABOUT Amaze HD Conditions of Experiment Column: Amaze HD Separation Modes: HILIC, anion-exchange Column Dimenstions: 3.0x100 mm, 3 um, 100A Mobile Phase: 85% ACN with 20 mM Ammonium Acetate pH 4 Detection: 280 nm Sample: 0.2 mg/ml Injection: 2 uL Flow rate: 0.6 ml/min Analytes Class of compounds: Aromatic acid, Organic acid, Supplement, Vitamin Nature of compounds: Acidic, Hydrophilic, Polar Compounds: Isoascorbic acid, Ascorbic acid HPLC Separation of Ascorbic Acid and Ascorbic Acid-2-Phosphate in HILIC and Anion-Exclusion Modes on Amaze HD Column Amaze HD PURCHASE LEARN ABOUT Amaze HD Conditions of Experiment Column: Amaze HD Separation Modes: HILIC and anion-exclusion Column Dimenstions: 3.0x100 mm, 3 um, 100A Mobile Phase: from 85% ACN with 30 mM Ammonium Acetate pH 4to 50% ACN with 10 mM Ammonium Acetate pH 4 in 10 min Detection: 280 nm Sample: 0.2 mg/ml Injection: 2 uL Flow rate: 0.6 ml/min Analytes Class of compounds: Aromatic acid, Metabolite, Organic acid, Supplement, Vitamin Nature of compounds: Acidic, Hydrophilic, Polar Compounds: Ascorbic acid, Ascorbic acid 2-phosphate HPLC Separation of 15 Organic Acids on Amaze HA Mixed-Mode Column with a Triple Gradient Amaze HA PURCHASE LEARN ABOUT Amaze HA Conditions of Experiment Column: Amaze HA Separation Modes: reveresed-phase , anion-exchange Column Dimenstions: 4.6x100, 3 um, 100A Mobile Phase: "A: 2% ACN with 40 mM ammonium phosphate pH 4.0 B: 20% ACN with 100 mM ammonium phosphate pH 2.0 gradient from 100% A to 100% B in 15 minutes, 5 minutes hold " Detection: 205 nm Sample: 0.2-0.5 mg/ml Injection: 2 uL Flow rate: 1 ml/min Analytes Class of compounds: Aromatic acid, Isomer, Organic acid, Organic compound, Preservative, Supplement, Vitamin Nature of compounds: Acidic, Hydrophilic, Hydrophobic, Neutral, Polar Compounds: Acetic acid, Ascorbic acid, Formic acid, Succinic acid, Citric acid, Malonic acid, Fumaric acid, Mandelic acid, Oxalic acid, Terephthalic acid, Benzoic acid, Maleic acid, Phthalic acid, Benzenesulfonic acid, p-Toluenesulfonic acid HPLC Analysis of Ascorbic, Citric and Ethylenediaminetetraacetic acids on Heritage MA Mixed-Mode Column Heritage MA PURCHASE LEARN ABOUT Heritage MA Conditions of Experiment Column: Heritage MA Separation Modes: reversed-phase and anion-exchange Column Dimenstions: 4.6x50 mm, 3 μm, 100A Mobile Phase: ACN gradient from 2% to 40%, phosphoric acid gradient from 0.03% to 0.4% in 5 min, 2 min hold Detection: UV 220 nm Sample: 1 mg/ml Injection: 2 μL Flow rate: 1 ml/min Analytes Class of compounds: Aromatic acid, Organic acid, Preservative, Supplement, Vitamin Nature of compounds: Acidic, Hydrophilic, Polar, Zwitterionic Compounds: Ascorbic acid, Citric acid, Ethylenediaminetetraacetic acid HPLC Analysis of 10 Water-Soluble Vitamins in Reversed-Phase, Cation- and Anion-Exchange Modes on Amaze QR Tri-Modal Column Amaze QR PURCHASE LEARN ABOUT Amaze QR Conditions of Experiment Column: Amaze QR Separation Modes: reversed-phase, cation-exchange, anion-exchange Column Dimenstions: 4.6x150 mm, 3 μm, 100A Mobile Phase: ACN from 10% to 75%, AmFM pH 3 from 10 mM 50 mM in 12 min Detection: UV 275 nm, ELSD Sample: 1 ml/min Injection: 3 μL Flow rate: 1 ml/min Analytes Class of compounds: Amines, Aromatic acid, Aromatic base, Organic acid, Supplement, Vitamin Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Polar Compounds: Ascorbic acid, Pyridoxal, Pyridoxine, Pantothenic acid, Niacin, Cyanocobalamin, Riboflavin, Biotin, Folic acid, Thiamine HPLC Analysis of 10 Water-Soluble Vitamins in HILIC, Cation- and Anion-Exchange Modes on Amaze TH Tri-Modal Column Amaze TH PURCHASE LEARN ABOUT Amaze TH Conditions of Experiment Column: Amaze TH Separation Modes: reversed-phase, cation-exchange, anion-exchange Column Dimenstions: 4.6x150 mm, 3 μm, 100A Mobile Phase: ACN from 87% to 40%, AmFM pH 3 from 10 mM 60 mM in 10 min, 7 min hold Detection: UV 275 nm, ELSD Sample: 1 ml/min Injection: 3 μL Flow rate: 1 ml/min Analytes Class of compounds: Amines, Aromatic acid, Aromatic base, Organic acid, Supplement, Vitamin Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Polar Compounds: Nicotinamide, Biotin, Pantothenic acid, Riboflavin, Pyridoxine, Ascorbic acid, Niacin, Folic acid, Cyanocobalamin, Thiamine HPLC Analysis of Ascorbic and Citric Acids in HILIC/Anion-Exclusion Modes on Amaze HD Column Amaze HD PURCHASE LEARN ABOUT Amaze HD Conditions of Experiment Column: Amaze HD Separation Modes: HILIC, anion-exclusion Column Dimenstions: 4.6x150 mm, 3 μm, 100A Mobile Phase: 90% ACN, no additives Detection: 210 nm Sample: 1 ml/min Injection: 3 μL Flow rate: 1 ml/min Analytes Class of compounds: Aromatic acid, Organic acid, Preservative, Supplement, Vitamin Nature of compounds: Acidic, Hydrophilic, Polar Compounds: Citric acid, Ascorbic acid HPLC Analysis of Ascorbic and Citric Acids on Amaze TH Mixed-Mode Column Application description Ascorbic acid is a vitamin and preservative. Citric acid is a preservative. Both are widely used in food industry. Both acids are polar and acidic in nature. Retention on a regular C18 column can be achieved with addition of ion-pairing reagent. We have developed a simple and robust method for separation of ascorbic and citric acid in HILIC/anion-exchange mode on Amaze TH HPLC column. Method is using volatile mobile phase which is compatible with MS spectrometry. Method can be used for analysis of ascorbic and citric acids in various sample matrices including biofluids and complex food food matrices. Amaze TH column can retain compounds by combination of HILIC, cation- and anion-exchange. Amount of ACN, buffer concentration and buffer pH will affect retention of polar and ionizable compounds. Amaze TH PURCHASE LEARN ABOUT Amaze TH Conditions of Experiment Column: Amaze TH Separation Modes: HILIC, anion-exchange Column Dimenstions: 4.6 x 150 mm, 5 um, 100A, Mobile Phase: 75% ACN with 25 mM AmAc pH 4.2 Detection: ELSD Sample: 0.3 mg/ml Injection: 3 uL Flow rate: 1 ml/min Analytes Class of compounds: Aromatic acid, Organic acid, Preservative, Supplement, Vitamin Nature of compounds: Acidic, Hydrophilic, Polar Compounds: Ascorbic acid, Citric acid HPLC Analysis of Ascorbic, Methylmalonic and Succinic Acids on Heritage N Column Application description Ascorbic, succinic and malonic acid are weak organic acids. Ascorbic acid is a vitamin found in various foods. It is one of the more important supplements used in various formulations. Food containing Vitamin C includes fruits and vegetables. It is a very important nutrient for humans and animals. Vitamin C functions as a cofactor in many enzymatic reactions in humans and animals. It mediates a variety of essential biological functions like wound healing and collagen synthesis. Methylmalonic acid is a dicarboxylic acid. Succinic acid is a dicarboxylic acid which is used in the production of some polymers and component of some alkyl resins. It is also used as a food additive and dietary supplement. It serves as an acidity regulator. We have developed an HILIC anion-exclusion approach for the analysis of these three organic acids on Heritage N HPLC column. The retention time is controlled by the amount of ACN and the amount of buffer. No ion-pairing reagent is required. Various buffers can be used depending on your detection technique Heritage N PURCHASE LEARN ABOUT Heritage N Conditions of Experiment Column: Heritage N Separation Modes: HILIC, anion-exclusion Column Dimenstions: 4.6 x 150 mm, 5 um, 100A Mobile Phase: 75% ACN with 15 mM AmAc pH 7 Detection: ELSD Sample: 0.3 mg/ml Injection: 5 uL Flow rate: 1 ml/min Analytes Class of compounds: Aromatic acid, Organic acid, Supplement, Vitamin Nature of compounds: Acidic, Hydrophilic, Polar Compounds: Ascorbic acid, Methylmalonic acid, Succinic acid HPLC Analysis of Ascorbic and Dehydroascorbic acids on Coresep SB Column in reversed-Phase Anion-Exchange Modes Application description Ascorbic acid, or Vitamin C, is a vitamin found in plants, fruits and other food-related sources. It is one of the main dietary supplements. Vitamin C is an essential nutrient involved in repair of tissues. It is available as generic over-the-counter medication. Dehydroascorbic acid is an oxidized form of ascorbic acid. Both ascorbic and dehydroascorbic acids have similar biological activity as antiviral medications, but dehydroascorbic acid also has neuroprotective effects. Both compounds are very polar, with limited or no retention in reversed-phase chromatography. Both acids were analyzed and retained by weak reversed-phase and anion-exchange mechanisms on a core-shell mixed-mode anion-exchange column. The method and column can be used for analysis of these and other hydrophilic acids by mixed-mode chromatography. The method is compatible with LC/MS, ELSD and CAD and can be used for quantitation of ascorbic and dehydroascorbic acids in various foods, drug formulations and biofluids. Coresep SB column combines unique selectivity of mixed-mode with ultra-high efficiency of core-shell particles technology. HPLC method is reproducible and robust. Coresep SB PURCHASE LEARN ABOUT Coresep SB Conditions of Experiment Column: Coresep SB Separation Modes: reversed-phase and anion-exchange Column Dimenstions: 3.2 x 100 mm, 2.7 um, 90A Mobile Phase: 5% ACN with 0.05% of formic acid Detection: UV 250 nm Sample: 0.5 mg/ml Injection: 1 uL Flow rate: 0.8 ml/mim Analytes Class of compounds: Aromatic acid, Organic acid, Supplement, Vitamin Nature of compounds: Acidic, Hydrophilic, Polar Compounds: Dehydroascorbic acid, Ascorbic acid
