Benzoic acid
Column: | Amaze C18 |
Separation Modes: | reversed-phase |
Column Dimenstions: | 4.6x150 mm, 3 um, 100A |
Mobile Phase: | ACN/water/Ammonium phosphate |
Detection: | 220 nm |
Sample: | 0.3-0.5 mg/ml |
Injection: | 1 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Aldehyde, Aromatic acid, Aromatic compound, Organic acid, Organic compound, Preservative |
Nature of compounds: | Acidic, Hydrophilic, Hydrophobic, Neutral |
Compounds: | Benzyl alcohol, Phenol, Benzoic acid, Methylparaben, Benzonitrile, Ethylparaben, Propylparaben |
Column: | Amaze C18 |
Separation Modes: | reversed-phase |
Column Dimenstions: | 4.6x150 mm, 3 um, 100A |
Mobile Phase: | ACN/water/Ammonium phosphate |
Detection: | 220 nm |
Sample: | 0.3-0.5 mg/ml |
Injection: | 1 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Aldehyde, Aromatic acid, Aromatic compound, Organic acid, Organic compound, Preservative |
Nature of compounds: | Acidic, Hydrophilic, Hydrophobic, Neutral |
Compounds: | Benzyl alcohol, Phenol, Benzoic acid, Methylparaben, Benzonitrile, Ethylparaben, Propylparaben |
Column: | Amaze HA |
Separation Modes: | reveresed-phase , anion-exchange |
Column Dimenstions: | 4.6x100, 3 um, 100A |
Mobile Phase: | "A: 2% ACN with 40 mM ammonium phosphate pH 4.0 B: 20% ACN with 100 mM ammonium phosphate pH 2.0 gradient from 100% A to 100% B in 15 minutes, 5 minutes hold " |
Detection: | 205 nm |
Sample: | 0.2-0.5 mg/ml |
Injection: | 2 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Aromatic acid, Isomer, Organic acid, Organic compound, Preservative, Supplement, Vitamin |
Nature of compounds: | Acidic, Hydrophilic, Hydrophobic, Neutral, Polar |
Compounds: | Acetic acid, Ascorbic acid, Formic acid, Succinic acid, Citric acid, Malonic acid, Fumaric acid, Mandelic acid, Oxalic acid, Terephthalic acid, Benzoic acid, Maleic acid, Phthalic acid, Benzenesulfonic acid, p-Toluenesulfonic acid |
Column: | Amaze SC |
Separation Modes: | RP, anion-exclusion |
Column Dimenstions: | 3.0x100 mm, 3 μm, 100A |
Mobile Phase: | ACN/water/phosphoroic acid |
Detection: | 255 nm |
Sample: | 0.3 mg/ml |
Injection: | 2 μL |
Flow rate: | 0.6 ml/min |
Class of compounds: | Aromatic acid, Organic acid |
Nature of compounds: | Acidic, Hydrophilic |
Compounds: | 4-Carboxyphenylboronic acid, Benzoic acid |
Comparison run on Amaze PFP, Amaze AR, Amaze SPF, and Heritage C20 columns.
Column: | Amaze SPF |
Separation Modes: | reversed-phase, pi-pi |
Column Dimenstions: | 4.6x150 mm, 5 μm, 100A |
Mobile Phase: | 35% ACN with 20 mM AmAc pH 4.5 |
Detection: | UV 235 nm |
Sample: | 1 mg/ml |
Injection: | 3 μL |
Flow rate: | 1 ml/min |
Class of compounds: | Amines, Aromatic acid, Aromatic base, Aromatic compound, Organic acid, Organic compound, Preservative |
Nature of compounds: | Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar |
Compounds: | 4-Aminophenol, Mandelic acid, 4-Hydroxybenzoic acid, Phenol, Benzoic acid, Methylparaben |
Comparison run on Amaze PFP, Amaze AR, Amaze SPF, and Heritage C20 columns.
Column: | Amaze AR |
Separation Modes: | reversed-phase, pi-pi |
Column Dimenstions: | 4.6x150 mm, 5 μm, 100A |
Mobile Phase: | 28% ACN with 20 mM AmAc pH 4.5 |
Detection: | UV 255 nm |
Sample: | 1 mg/ml |
Injection: | 3 μL |
Flow rate: | 1 ml/min |
Class of compounds: | Amino acid, Aromatic acid, Aromatic compound, Drug, Isomer, Organic acid, Preservative, Stimulant, Xanthine |
Nature of compounds: | Acidic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic |
Compounds: | Caffeine, 4-Aminobenzoic acid, Aspirin, 4-Hydroxybenzoic acid, Sorbic acid, Benzoic acid |
Comparison run on Amaze PFP, Amaze AR, Amaze SPF, and Heritage C18 columns.
Column: | Amaze PFP |
Separation Modes: | reversed-phase, pi-pi |
Column Dimenstions: | 4.6x150 mm, 5 μm, 100A |
Mobile Phase: | 50% ACN with 20 mM AmFm pH 3 |
Detection: | UV 255 nm |
Sample: | 1 mg/ml |
Injection: | 3 μL |
Flow rate: | 1 ml/min |
Class of compounds: | Aromatic acid, Aromatic compound, Organic acid, Organic compound, Organic solvent |
Nature of compounds: | Acidic, Hydrophilic, Hydrophobic, Neutral, Polar |
Compounds: | Benzyl alcohol, Phenol, Benzoic acid, Toluene, Benzenesulfonic acid |
Seven compounds with different properties were used to study an effect of different acids in the mobile phase on retention of neutral, acidic, basic and zwitterionic compounds on core-shell mixed-mode Coresep 100 column. Retention of neutral compounds (propylparaben and benzoic acid to some extent) are not affected by change of the acid. Basic compounds (norphenylephrine, pyridine, 2-aminopyridine) respond the most to change of the strength of acidic additive. Several folds increase in retention time was observed when sulfuric acid was replaced with formic acid in the mobile phase. Effect of acid on retention time of zwitterionic amino acids was less pronounced but still provided excellent retention and peak shape for zwitterionic compounds (5-aminosalicylic acid, phenylalanine). This methods and Coresep 100 column can be used to retain and separate other acidic, basic, neutral and zwitterionic compounds without the use of ion-pairing reagent in the mobile phase. Various detection techniques can be used based on the properties of the mobile phase and analytes. Methods are compatible with mall major detection techniques 9UV, ELSD, CAD, RI, MS). Core-shell mixed-mode columns provide great combination of unique selectivity, speed and efficiency.
Column: | Coresep 100 |
Separation Modes: | reversed-phase, cation-exchange |
Column Dimenstions: | 4.6 x 150 mm, 2.7 um, 90A |
Mobile Phase: | 30% ACN with various acids |
Detection: | UV 255 nm |
Sample: | 0.3 mg/ml |
Injection: | 3 uL |
Flow rate: | 1 mL/min |
Class of compounds: | Amines, Amino acid, Aromatic acid, Aromatic base, Aromatic compound, Catecholamine, Drug, Isomer, Neurotransmitter, Organic acid, Preservative, Pyridine, Supplement |
Nature of compounds: | Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic |
Compounds: | 5-Aminosalicylic acid, Norphenylephrine, Phenylalanine, Pyridine, Benzoic acid, 2-Aminopyridine, Propylparaben |
Column: | Heritage С18 |
Separation Modes: | reversed-phase |
Column Dimenstions: | 4.6 x 100 mm, 5 um, 100A |
Mobile Phase: | MeOH/H2O/AcOH 30/67/3 |
Detection: | UV 275 nm |
Sample: | 0.2 mg/ml |
Injection: | 5 uL |
Flow rate: | 1 mL/min |
Class of compounds: | Aromatic acid, Aromatic compound, Drug, Organic acid, Stimulant, Xanthine |
Nature of compounds: | Acidic, Hydrophilic, Hydrophobic, Neutral, Polar |
Compounds: | Acetaminophen, Caffeine, Aspirin, Benzoic acid, Salicylic acid |
Column: | Heritage С18 |
Separation Modes: | reversed-phase |
Column Dimenstions: | 4.6 x 150 mm, 5 um, 100A |
Mobile Phase: | MeOH/H2O/AcOH 40/60/1 |
Detection: | UV 254 nm |
Sample: | 0.3 mg/ml |
Injection: | 5 uL |
Flow rate: | 1 mL/min |
Class of compounds: | Aromatic acid, Aromatic compound, Drug, Organic acid |
Nature of compounds: | Acidic, Hydrophilic, Neutral, Polar |
Compounds: | Guaifenesin, Benzoic acid |
Core-shell mixed-mode columns offer unique selectivity which comes from the presence of two mechanisms - reversed-phase and ion-exchange. The alternative selectivity of mixed-mode columns can be used when order of elution or resolution between compounds needs improvement. Even for neutral compounds a different selectivity can be obtained. A method for common components of headache medications (acetaminophen, caffeine, and benzoate) was developed on our Coresep 100 column. Efficiency and peak shape are comparable to leading brand core-shell reversed-phase columns.
Column: | Coresep 100 |
Separation Modes: | reversed-phase |
Column Dimenstions: | 3.2 x 50 mm, 2.7 um, 90A |
Mobile Phase: | 20% ACN with 0.1% TFA |
Detection: | UV 250 nm, ELSD |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Aromatic acid, Aromatic compound, Drug, Organic acid, Stimulant, Xanthine |
Nature of compounds: | Acidic, Hydrophilic, Neutral |
Compounds: | Acetaminophen, Caffeine, Benzoic acid |
Mixture of hydrophilic acidic compounds are separated in reversed-phase anion-exchange mode on core-shell mixed-mode column. Method is robust, reliable and fast and can be used for analysis of underivatized acids without ion-pairing reagent.
Column: | Coresep SB |
Separation Modes: | reversed-phase and anion-exchange |
Column Dimenstions: | 3.2 x 100 mm, 2.7 um, 90A |
Mobile Phase: | 50% ACN with 50 mM sodium phosphate (monobasic) pH 3 |
Detection: | UV 230 nm |
Sample: | 0.3 mg/ml |
Injection: | 3 uL |
Flow rate: | 0.5 mg/ml |
Class of compounds: | Aromatic acid, Organic acid |
Nature of compounds: | Acidic, Hydrophilic, Polar |
Compounds: | Benzoic acid, Maleic acid, Fumaric acid |