Diethanolamine
HPLC Separation of Ethanolamines on Amaze SC Mixed-Mode Column
Conditions of Experiment
Column: | Amaze SC |
Separation Modes: | reversed-phase cation-exchange |
Column Dimenstions: | 3.0x100 mm, 5 μm, 100A |
Mobile Phase: | 2% ACN with 0.1% TFA |
Detection: | Corona CAD |
Sample: | 0.5 mg/ml |
Injection: | 2 μL |
Flow rate: | 0.6 ml/min |
Analytes
Class of compounds: | Amines, Ethanolamine |
Nature of compounds: | Basic, Hydrophilic, Polar |
Compounds: | Ethanolamine, Diethanolamine, Triethanolamine |
HPLC Analysis of Ethanolamines
Application description
Ethanolamines are commonly used basic compounds which often act as surfactants and cleansing agents. Diethanolamine for example, is used for inhibiting corrosion, industrial gas scrubbing to remove hydrogen sulfide from gas, and used in production of morpholine. Coresep 100 was used to separate monoethanolamine, diethanolamine, triethanolamine, and morpholine, a pH adjusting agent used in the petroleum industry synthesized from diethanolamine. Coresep 100 is a mixed-mode column that retains bases using cation-exchange. Ethanolamines are volatile and have no UV activity. A refraction index detector was used to analyze ethanolamines.
Conditions of Experiment
Column: | Coresep 100 |
Separation Modes: | reversed-phase and cation-exchange |
Column Dimenstions: | 3.2 x 50 mm, 2.7 um, 90A |
Mobile Phase: | 2% of ACN with 5 mM AmAc pH 3.0 |
Detection: | RI |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 0.6 ml/min |
Analytes
Class of compounds: | Amines, Ethanolamine |
Nature of compounds: | Basic, Hydrophilic, Polar |
Compounds: | Ethanolamine, Diethanolamine, Triethanolamine, Morpholine |