Dopamine
Column: | Amaze HD |
Separation Modes: | HILIC, cation-exchange and anion-exclusion |
Column Dimenstions: | 3.0x100 mm, 3 μm, 100A |
Mobile Phase: | 72% ACN with 56 mM AmFm pH 3 |
Detection: | Corona CAD |
Sample: | 1 mg/ml |
Injection: | 2 μL |
Flow rate: | 0.6 ml/min |
Class of compounds: | Amino acid, Aromatic acid, Catecholamine, Drug, Inorganic anion, Neurotransmitter, Supplement |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar, Zwitterionic |
Compounds: | Chloride Ion, Citrulline, 4-Aminobuyric acid (GABA), Ornithine, Dopamine, Arginine |
Column: | Amaze SC |
Separation Modes: | reversed-phase and cation-exchange |
Column Dimenstions: | 3.0x100 mm, 5μm, 100A |
Mobile Phase: | 10% ACN with 20 mM AmFm pH 3 |
Detection: | Corona CAD |
Sample: | 0.5 mg/ml |
Injection: | 2 μL |
Flow rate: | 0.6 ml/min |
Class of compounds: | Amino acid, Aromatic acid, Catecholamine, Drug, Inorganic anion, Neurotransmitter, Supplement |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar, Zwitterionic |
Compounds: | Chloride Ion, Citrulline, 4-Aminobuyric acid (GABA), Ornithine, Dopamine, Arginine |
Column: | Amaze SC |
Separation Modes: | reversed-phase cation-exchange |
Column Dimenstions: | 3.0x100 mm, 5 μm, 100A |
Mobile Phase: | 40% ACN with 40 mM AmFm pH 3 |
Detection: | UV 250 nm |
Sample: | 0.3 mg/ml |
Injection: | 2 μL |
Flow rate: | 0.6 ml/min |
Class of compounds: | Aromatic base, Catecholamine, Drug, Neurotransmitter, Stimulant |
Nature of compounds: | Basic, Hydrophilic, Polar |
Compounds: | Epinephrine, Norepinephrine, Dopamine, Ephedrine |
Amino acids are essential components of numerous formulation. Health supplements can contain various amino acids and vitamins and require quantitation of each ingredients. Amino acids are very polar compounds with limited or no retention in reversed-phase chromatography. The most common approaches are reversed-phase chromatography with ion-pairing reagent and hydrophilic interaction chromatography (HILIC). Underivatized amino acids can be retained by combination of reversed-phase and cation exchange mechanism. These two mechanism were combined in Coresep 100 core-shell mixed-mode column. Method can be used for fast analysis of underivatized amino acids without ion-pairing reagent. Depending on the pH of the mobile phase they can exist in acidic, basic or zwitter-ionic form. At lower pH, amino acids are basic in nature and have the highest hydrophobicity. Four amino acids (SER, GLY, ALA, VAL and DOPA) were retained and separated within 2 minutes on core-shell mixed-mode column. This high efficiency separation does not require special UPLC set up and can benefit from core-shell and mixed-mode approaches.
Column: | Coresep 100 |
Separation Modes: | reversed-phase and cation-exchange |
Column Dimenstions: | 3.2 x 50 mm, 2.7 um, 90A |
Mobile Phase: | 3% ACN with 0.03% TFA |
Detection: | UV 210 nm, ELSD |
Sample: | 0.3 mg/ml |
Injection: | 10 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Amino acid, Catecholamine, Drug, Neurotransmitter, Supplement |
Nature of compounds: | Basic, Hydrophilic, Polar, Zwitterionic |
Compounds: | Glycine, Alanine, Serine, Valine, Dopamine |
Catecholamines (neurotransmitters) are derivatives of the amino acid tyrosine. DOPA, tyrosine, phenylalanine, norepinephrine, epinephrine, and dopamine. Fast and efficient method with base-line separation was achieved on Coresep 100 column. All compounds are separated by combination of reversed-phase and cation-exchange mechanisms. Peak order and retention time can be changed by switching from TFA to ammonium formate in the mobile phase, by adjusting mobile phase composition and by changing pH. The method is fully compatible with mass spectroscopy and can be used for fast analysis of neurotransmitters in biofluids. regular. Method can be used a replacement for analysis of neurotransmitters by UPLC with ion-pairing reagents.
Column: | Coresep 100 |
Separation Modes: | reversed-phase and cation-exchange |
Column Dimenstions: | 3.2 x 100 mm, 2.7 um, 90A |
Mobile Phase: | ACN gradient from 5% to 10% in 5 min, buffer gradient - Ammonium formate pH 2.9 from 5 to 25 mM in 5 min |
Detection: | UV 270 nm, ELSD |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Amino acid, Aromatic base, Catecholamine, Drug, Neurotransmitter, Supplement |
Nature of compounds: | Basic, Hydrophilic, Polar, Zwitterionic |
Compounds: | Dopamine, Tyrosine, Phenylalanine, Norepinephrine, Epinephrine, DOPA |