Methionine
Column: | Amaze SC |
Separation Modes: | reversed-phase cation-exchange |
Column Dimenstions: | 4.6x250 mm, 5 μm, 100A |
Mobile Phase: | A: 2% ACN with 0.1% TFA B: 25% ACN with 0.3% TFA from 100% A to 100% B in 20 min, 8 min hold |
Detection: | Corona CAD |
Sample: | 0.2 mg/ml |
Injection: | 5 μL |
Flow rate: | 1 ml/min |
Class of compounds: | Amino acid, Neurotransmitter, Supplement |
Nature of compounds: | Acidic, Hydrophilic, Polar, Zwitterionic |
Compounds: | Aspartic acid, Serine, Asparagine, Cysteine, Glutamic acid, Threonine, Glutamine, Glycine, Alanine, Proline, Valine, Methionine, Tyrosine, Isoleucine, Leucine, Phenylalanine, Lysine, Histidine, Tryptophan, Arginine |
Column: | Amaze HD |
Separation Modes: | HILIC, cation-exchange |
Column Dimenstions: | 3.0x100 mm, 5 um, 100A |
Mobile Phase: | 80% ACN with 20 mM AmFm pH 3 |
Detection: | ELSD, 45°C |
Sample: | 0.5-0.8 mg/ml |
Injection: | 3 uL |
Flow rate: | 0.6 ml/min |
Class of compounds: | Amino acid, Catecholamine, Drug, Neurotransmitter, Supplement |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar, Zwitterionic |
Compounds: | Tryptophan, Phenylalanine, Leucine, Methionine, Tyrosine, Isoleucine, DOPA, Valine, Cysteine, Proline, Alanine, Threonine, Glutamic acid, Glycine, Glutamine, Asparagine, Serine, Aspartic acid |
Amino acids are very polar compounds which are used as a building blocks in pharmaceutical industry. They are widely used as supplements and food additives. Depending on the pH of the mobile phase they can exist in acidic, basic or zwitter-ionic form. At pH below 3, amino acids are basic in nature and have the highest hydrophobicity. Within pH of 3 to 7 amino acids are in zwitter-ionic form where they are the most hydrophilic. There is no mechanism of retention on reversed-phase column and amino acids are not retained. Bufferless ion-separation (BLIS) was introduced by SIELC as a way to retain and analyze amino acids in reversed-phase cation-exchange mode. This mode allows to analyze amino acids as zwitter-ions without any ions/buffers in the mobile phase. The method was adopted for analysis of amino acids on core-shell mixed-mode columns. A similar approach on a reversed-phase core-shell column results in distorted peaks and no separation or significant retention to achieve baseline separation.
Column: | Coresep 100 |
Separation Modes: | reversed-phase and cation-exchange |
Column Dimenstions: | 3.2 x 50 mm, 2.7 um, 90A |
Mobile Phase: | 20% ACN with no additives |
Detection: | UV 205 nm, ELSD |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Amino acid, Supplement |
Nature of compounds: | Hydrophilic, Polar, Zwitterionic |
Compounds: | Methionine, Tyrosine, Phenylalanine |