Norepinephrine
Column: | Amaze SC |
Separation Modes: | reversed-phase cation-exchange |
Column Dimenstions: | 3.0x100 mm, 5 μm, 100A |
Mobile Phase: | 40% ACN with 40 mM AmFm pH 3 |
Detection: | UV 250 nm |
Sample: | 0.3 mg/ml |
Injection: | 2 μL |
Flow rate: | 0.6 ml/min |
Class of compounds: | Aromatic base, Catecholamine, Drug, Neurotransmitter, Stimulant |
Nature of compounds: | Basic, Hydrophilic, Polar |
Compounds: | Epinephrine, Norepinephrine, Dopamine, Ephedrine |
Column: | Heritage С18 |
Separation Modes: | reversed-phase |
Column Dimenstions: | 4.6 x 150 mm, 5 um, 100A |
Mobile Phase: | Sodium heptanesulfonate/H2O/MeOH 0.20/80/20 pH 3.0 |
Detection: | UV 270 nm |
Sample: | 0.3 mg/ml |
Injection: | 5 uL |
Flow rate: | 1 mL/min |
Class of compounds: | Aromatic base, Catecholamine, Neurotransmitter |
Nature of compounds: | Basic, Hydrophilic, Polar |
Compounds: | Norepinephrine |
Four neurotransmitters - pseudoephedrine, norephedrine, phenylephrine and norphenylephrine - were separated in hydrogen-bonding and cation-exchange mode. Pseudoephedrine is sympathomimetic drug used either as a single ingredient or part of the composition of OTC or Rx medications. Phenylephrine is a hydrophilic basic drug which is used as decongestant in OTC medication composition, such as Sudafed, Benadryl, Advil, Nyquil, etc. Norphenylephrine is an adrenergic agent used as sympathomimetic drug. Norephedrine is naturally occurring endogenous trace amine that plays a role as neurotransmitter in the brain, and is used as decongestant in many OTC compositions as well as an appetite suppressant. All four drugs are hydrophilic and basic in nature, and have no retention on traditional reversed-phase column without an ion-pairing reagent. Method provides an efficient and robust separation for all drugs. The method can be used in analysis of neurotransmitters in biofluids and other sample matrices. The method is compatible with all detection techniques: MS, RI, UV, ELSD and CAD.
Column: | Amaze HD |
Separation Modes: | hydrogen-bonding and cation-exchange |
Column Dimenstions: | 3.2 x 50 mm, 3 um |
Mobile Phase: | MeCN/MeOH (95/5) with 0.5% HCOOH and 0.05% AmFm |
Detection: | UV 275 nm |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 1.0 mL/min |
Class of compounds: | Amino acid, Aromatic base, Catecholamine, Drug, Neurotransmitter, Stimulant, Supplement |
Nature of compounds: | Basic, Hydrophilic, Polar, Zwitterionic |
Compounds: | Pseudoephedrine, Norepinephrine, Phenylalanine, Norphenylephrine |
Catecholamines (neurotransmitters) are derivatives of the amino acid tyrosine. DOPA, tyrosine, phenylalanine, norepinephrine, epinephrine, and dopamine. Fast and efficient method with base-line separation was achieved on Coresep 100 column. All compounds are separated by combination of reversed-phase and cation-exchange mechanisms. Peak order and retention time can be changed by switching from TFA to ammonium formate in the mobile phase, by adjusting mobile phase composition and by changing pH. The method is fully compatible with mass spectroscopy and can be used for fast analysis of neurotransmitters in biofluids. regular. Method can be used a replacement for analysis of neurotransmitters by UPLC with ion-pairing reagents.
Column: | Coresep 100 |
Separation Modes: | reversed-phase and cation-exchange |
Column Dimenstions: | 3.2 x 100 mm, 2.7 um, 90A |
Mobile Phase: | ACN gradient from 5% to 10% in 5 min, buffer gradient - Ammonium formate pH 2.9 from 5 to 25 mM in 5 min |
Detection: | UV 270 nm, ELSD |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Amino acid, Aromatic base, Catecholamine, Drug, Neurotransmitter, Supplement |
Nature of compounds: | Basic, Hydrophilic, Polar, Zwitterionic |
Compounds: | Dopamine, Tyrosine, Phenylalanine, Norepinephrine, Epinephrine, DOPA |