Terephthalic acid

HPLC Separation of 15 Organic Acids on Amaze HA Mixed-Mode Column with a Triple Gradient
Conditions of Experiment
Column: Amaze HA
Separation Modes: reveresed-phase , anion-exchange
Column Dimenstions: 4.6x100, 3 um, 100A
Mobile Phase: "A: 2% ACN with 40 mM ammonium phosphate pH 4.0 B: 20% ACN with 100 mM ammonium phosphate pH 2.0 gradient from 100% A to 100% B in 15 minutes, 5 minutes hold "
Detection: 205 nm
Sample: 0.2-0.5 mg/ml
Injection: 2 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Aromatic acid, Isomer, Organic acid, Organic compound, Preservative, Supplement, Vitamin
Nature of compounds: Acidic, Hydrophilic, Hydrophobic, Neutral, Polar
Compounds: Acetic acid, Ascorbic acid, Formic acid, Succinic acid, Citric acid, Malonic acid, Fumaric acid, Mandelic acid, Oxalic acid, Terephthalic acid, Benzoic acid, Maleic acid, Phthalic acid, Benzenesulfonic acid, p-Toluenesulfonic acid
HPLC Separation of Isomers of Phthalic Acid on Heritage MA Mixed-Mode Column
Conditions of Experiment
Column: Heritage MA
Separation Modes: reversed-phase and anion-exchange
Column Dimenstions: 4.6 x 50 mm
Mobile Phase: 40% ACN with 100 mM AmFm pH 3
Detection: UV 255 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1 mL/min
Analytes
Class of compounds: Aromatic acid, Isomer, Organic acid
Nature of compounds: Acidic, Hydrophilic, Hydrophobic, Polar
Compounds: Terephthalic acid, Isophthalic acid, Phthalic acid
HPLC Method for Analysis of Phthalic Acids on Coresep SB Column in Reversed-Phase and Anion-Exchange Modes
Application description

Phthalic acids are aromatic dicarboxylic acids with various positions of carboxylic groups. Isomers of phthalic acid were analyzed in reversed-phase anion-exchange on Coresep SB HPLC mixed-mode column. The small difference in hydrophobicity/polarity and acidic properties of molecules contributes to great resolution between these acids. Method and HPLC column can be used for analysis of phthalic and other acids in various matrices with various detection techniques (UV, ELSD, RI, MS, etc.). Coresep SB column combines unique selectivity of mixed-mode with ultra-high efficiency of core-shell particles technology. HPLC method is reproducible and robust.

Conditions of Experiment
Column: Coresep SB
Separation Modes: reversed-phase and anion-exchange
Column Dimenstions: 3.2 x 100 mm, 2.7 um, 90A
Mobile Phase: 50% ACN with 50 mM sodium phosphate (monobasic) pH 3
Detection: UV 230 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.8 ml/mim
Analytes
Class of compounds: Aromatic acid, Isomer, Organic acid
Nature of compounds: Acidic, Hydrophobic
Compounds: Phthalic acid, Terephthalic acid