Terephthalic acid
HPLC Separation of 15 Organic Acids on Amaze HA Mixed-Mode Column with a Triple Gradient
Conditions of Experiment
Column: | Amaze HA |
Separation Modes: | reveresed-phase , anion-exchange |
Column Dimenstions: | 4.6x100, 3 um, 100A |
Mobile Phase: | "A: 2% ACN with 40 mM ammonium phosphate pH 4.0 B: 20% ACN with 100 mM ammonium phosphate pH 2.0 gradient from 100% A to 100% B in 15 minutes, 5 minutes hold " |
Detection: | 205 nm |
Sample: | 0.2-0.5 mg/ml |
Injection: | 2 uL |
Flow rate: | 1 ml/min |
Analytes
Class of compounds: | Aromatic acid, Isomer, Organic acid, Organic compound, Preservative, Supplement, Vitamin |
Nature of compounds: | Acidic, Hydrophilic, Hydrophobic, Neutral, Polar |
Compounds: | Acetic acid, Ascorbic acid, Formic acid, Succinic acid, Citric acid, Malonic acid, Fumaric acid, Mandelic acid, Oxalic acid, Terephthalic acid, Benzoic acid, Maleic acid, Phthalic acid, Benzenesulfonic acid, p-Toluenesulfonic acid |
HPLC Separation of Isomers of Phthalic Acid on Heritage MA Mixed-Mode Column
Conditions of Experiment
Column: | Heritage MA |
Separation Modes: | reversed-phase and anion-exchange |
Column Dimenstions: | 4.6 x 50 mm |
Mobile Phase: | 40% ACN with 100 mM AmFm pH 3 |
Detection: | UV 255 nm |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 1 mL/min |
Analytes
Class of compounds: | Aromatic acid, Isomer, Organic acid |
Nature of compounds: | Acidic, Hydrophilic, Hydrophobic, Polar |
Compounds: | Terephthalic acid, Isophthalic acid, Phthalic acid |
HPLC Method for Analysis of Phthalic Acids on Coresep SB Column in Reversed-Phase and Anion-Exchange Modes
Application description
Phthalic acids are aromatic dicarboxylic acids with various positions of carboxylic groups. Isomers of phthalic acid were analyzed in reversed-phase anion-exchange on Coresep SB HPLC mixed-mode column. The small difference in hydrophobicity/polarity and acidic properties of molecules contributes to great resolution between these acids. Method and HPLC column can be used for analysis of phthalic and other acids in various matrices with various detection techniques (UV, ELSD, RI, MS, etc.). Coresep SB column combines unique selectivity of mixed-mode with ultra-high efficiency of core-shell particles technology. HPLC method is reproducible and robust.
Conditions of Experiment
Column: | Coresep SB |
Separation Modes: | reversed-phase and anion-exchange |
Column Dimenstions: | 3.2 x 100 mm, 2.7 um, 90A |
Mobile Phase: | 50% ACN with 50 mM sodium phosphate (monobasic) pH 3 |
Detection: | UV 230 nm |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 0.8 ml/mim |
Analytes
Class of compounds: | Aromatic acid, Isomer, Organic acid |
Nature of compounds: | Acidic, Hydrophobic |
Compounds: | Phthalic acid, Terephthalic acid |