Uridine
Column: | Amaze HA |
Separation Modes: | reversed-phase and anion-exchange |
Column Dimenstions: | 3.0x100 mm, 3 um, 100A |
Mobile Phase: | ACN/Water/Ammonium formate pH 3 |
Detection: | 275 nm |
Sample: | 0.2-0.4 mg/ml |
Injection: | 2 uL |
Flow rate: | 0.6 ml/min |
Class of compounds: | Aromatic acid, Nucleoside, Nucleotide |
Nature of compounds: | Acidic, Hydrophilic, Neutral |
Compounds: | Uridine, Uridine 5-monophosphate, Uridine 5-diphosphate, Uridine 5-triphosphate |
Column: | Amaze TCH |
Separation Modes: | HILIC, cation-exchange |
Column Dimenstions: | 4.6x150 mm, 3 um, 100A |
Mobile Phase: | ACN from 92% to 70% in 15 min, 10 mM AmAc pH 5 |
Detection: | UV 255 nm |
Sample: | 0.2-0.5 mg/ml |
Injection: | 2 uL |
Flow rate: | 1 ml/min |
Class of compounds: | Aromatic base, Nucleobase, Nucleoside |
Nature of compounds: | Basic, Hydrophilic, Neutral, Polar |
Compounds: | Thymine, Uracil, Thymidine, Uridine, Adenine, Adenosine, Cytosine, Inosine, Guanine, Cytidine, Guanosine |
Thymidine, uridine, deoxyadenosine, adenosine, deoxyguanosine and guanosine were separated on an Amaze HD column with LC/MS compatible method. The approach can be used to analyze nucleotides, nucleosides and their derivatives in various sample matrices. This HPLC method is robust and reproducible, and can be used for other compounds that can form hydrogen bonds with stationary phase and interact by electrostatic interaction.
Column: | Amaze HD |
Separation Modes: | hydrogen-bonding and cation-exchange |
Column Dimenstions: | 3.2 x 100 mm |
Mobile Phase: | MeCN/MeOH from 90/10 to 50/50 with 0.1% HCOOH and 0.01% AmFm in 8 min |
Detection: | UV 275 nm |
Sample: | 0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 0.8 mL/min |
Class of compounds: | Aromatic base, Nucleoside |
Nature of compounds: | Basic, Hydrophilic, Neutral, Polar |
Compounds: | Thymidine, Uridine, 2′-Deoxyadenosine, Adenosine, Guanosine |
Uracil and Uridine are nucleosides which occur as a part of RNA. Both compounds are neutral in nature and very polar. Uracil is often used as a void marker in reversed-phase HPLC. A fast HILIC-based method was developed for analysis of uracil and uridine on the core-shell HILIC and cation-exchange column. Method provides good peak shape and retention control. Column and method can be used for analysis of polar compounds in HILIC, and HILIC/mixed-mode.
Column: | Coresep S |
Separation Modes: | HILIC |
Column Dimenstions: | 3.2 x 100 mm, 2.7 um, 90A |
Mobile Phase: | 98% ACN with no additives |
Detection: | UV 250 nm |
Sample: | 0.1-0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 0.5 mg/ml |
Class of compounds: | Nucleobase, Nucleoside |
Nature of compounds: | Hydrophilic, Neutral |
Compounds: | Uracil, Uridine |