Fumaric acid
HPLC Separation of 15 Organic Acids on Amaze HA Mixed-Mode Column with a Triple Gradient
Conditions of Experiment
Column: | Amaze HA |
Separation Modes: | reveresed-phase , anion-exchange |
Column Dimenstions: | 4.6x100, 3 um, 100A |
Mobile Phase: | "A: 2% ACN with 40 mM ammonium phosphate pH 4.0 B: 20% ACN with 100 mM ammonium phosphate pH 2.0 gradient from 100% A to 100% B in 15 minutes, 5 minutes hold " |
Detection: | 205 nm |
Sample: | 0.2-0.5 mg/ml |
Injection: | 2 uL |
Flow rate: | 1 ml/min |
Analytes
Class of compounds: | Aromatic acid, Isomer, Organic acid, Organic compound, Preservative, Supplement, Vitamin |
Nature of compounds: | Acidic, Hydrophilic, Hydrophobic, Neutral, Polar |
Compounds: | Acetic acid, Ascorbic acid, Formic acid, Succinic acid, Citric acid, Malonic acid, Fumaric acid, Mandelic acid, Oxalic acid, Terephthalic acid, Benzoic acid, Maleic acid, Phthalic acid, Benzenesulfonic acid, p-Toluenesulfonic acid |
HPLC Analysis of Mono-, Di- and Tricarboxylic Acids on Amaze HA Mixed-Mode Column
Conditions of Experiment
Column: | Amaze HA |
Separation Modes: | reveresed-phase , anion-exchange |
Column Dimenstions: | 4.6x100, 3 um, 100A |
Mobile Phase: | ACN/water/phosphoric acid and ACN/water/ammonium phosphate buffer |
Detection: | 205 nm |
Sample: | 0.2-0.5 mg/ml |
Injection: | 2 uL |
Flow rate: | 1 ml/min |
Analytes
Class of compounds: | Organic acid, Preservative |
Nature of compounds: | Acidic, Hydrophilic, Polar |
Compounds: | Citric acid, Fumaric acid, Oxalic acid, Maleic acid, Acetic acid, Formic acid |
HPLC Analysis of Hydrophilic Acids on Coresep SB Column in Reversed-Phase and Anion-Exchange Modes
Application description
Mixture of hydrophilic acidic compounds are separated in reversed-phase anion-exchange mode on core-shell mixed-mode column. Method is robust, reliable and fast and can be used for analysis of underivatized acids without ion-pairing reagent.
Conditions of Experiment
Column: | Coresep SB |
Separation Modes: | reversed-phase and anion-exchange |
Column Dimenstions: | 3.2 x 100 mm, 2.7 um, 90A |
Mobile Phase: | 50% ACN with 50 mM sodium phosphate (monobasic) pH 3 |
Detection: | UV 230 nm |
Sample: | 0.3 mg/ml |
Injection: | 3 uL |
Flow rate: | 0.5 mg/ml |
Analytes
Class of compounds: | Aromatic acid, Organic acid |
Nature of compounds: | Acidic, Hydrophilic, Polar |
Compounds: | Benzoic acid, Maleic acid, Fumaric acid |