Quickly develop robust HPLC methods with our mixed-mode columns. Access the most comprehensive mixed-mode portfolio in the industry. Explore mixed-mode interactions. Benefit from enhanced retention, selectivity, and perfect peak shape.
New HPLC columns are introduced annually, many of which are merely slight modifications of existing models, typically focusing on a single interaction type, such as reversed-phase or HILIC. Often, these columns are designed by individuals lacking deep experience in solving separation challenges or in understanding the necessary modifications at the support surface. At HELIX Chromatography, with decades of experience since 2002, we have pioneered numerous stationary phases and recognize the need for a new approach to stationary phase design. We adhere to several critical design principles:
- Consistency in the surface modification process
- Chemical and mechanical robustness of the stationary phase and solid support
- Uniform particle size and distribution in the stationary support bed
- Reliable reproducibility in the packing process
- Optimal ligand density and surface area on the support
- Precise, single-ligand configurations to minimize analysis variability
Helix Chromatography Column Portfolio: Column Specifications and Applications Overview
Versatility:
Excellent
Column/Stationary Phase Description:
Tri-modal HILIC mixed-mode stationary phase with HILIC, cation-exchange and anion-exclusion properties. Hydrophilic short chain with an acidic ionizable group with pKa 3.
Mechanisms of Interaction:
HILIC, cation-exchange, and anion-exclusion.
Class of Analytes:
Amino acids, organic and inorganic bases, polar organic and inorganic acids, basic drugs, polar acidic drugs, environmental contaminants, sugars, and structural isomers.
Suggested Initial Mobile Phase Conditions and Buffers
70-80% ACN with 5-30 mM buffer pH 2-7. Buffers and additives: ammonium formate, ammonium acetate, and phosphate buffers at low concentrations.
Method Development Notes:
Explore different pH and buffer concentrations for different selectivities. Watch for solubility of phosphate buffers in high organic. Observe the recommended pH for the column. make sure buffers and additives are compatible with detection techniques. Make sure you consider significant cation-exchange capacity when changing buffers and equilibrating the column. The column is compatible with 100% ACN and 100% water. The column can operate in pure HILIC, pure cation-exchange, and anion-exclusion modes, and a combination of two or three modes.
Application Examples:
HPLC Separation of Mono-, Di- and Trisaccharides on Amaze HD HILIC Mixed-Mode Column
Separation of Fructose, Glucose and Sucrose on Amaze HD HILIC Mixed-Mode Column
HPLC Analysis of Dopamine and 5 Amino Acids in HILIC and Cation-Exchange Modes on Amaze HD Column
HPLC Analysis of Polar Environmental Contaminants on Amaze HD Mixed-Mode Column with LC/MS Compatible Conditions
More Applications
Versatility:
Very Good
Column/Stationary Phase Description:
Tri-modal HILIC mixed-mode stationary phase with HILIC, cation-exchange and anion-exchange properties. The acidic and basic groups are separated by a long hydrophilic linker. Acidic group pKa 3 basic group pKa 10.
Mechanisms of Interaction:
HILIC, cation-exchange, and anion-exchange.
Class of Analytes:
amino acids, organic and inorganic acids, basic and acidic drugs and corresponding counter-ions, environmental contaminants, sugars, and structural isomers.
Suggested Initial Mobile Phase Conditions and Buffers
70-80% ACN with 20-30 mM buffer pH 2-7. Buffers and additives: ammonium formate, ammonium acetate, phosphate buffers at low concentrations (5-10), and organic and inorganic acids.
Method Development Notes:
Explore different pH and buffer concentrations for different selectivities. Watch for solubility of phosphate buffers in high organic. Observe the recommended pH for the column. make sure buffers and additives are compatible with detection techniques. Make sure you consider significant cation- and anion-exchange capacity when changing buffers and equilibrating the column. The column can operate in pure HILIC, pure cation-exchange, and anion-exchange modes and a combination of two or three modes. The column is compatible with 100% ACN and 100% water.
Application Examples:
HPLC Analysis Four Amino Acids Used in Supplements on Amaze TH Mixed-Mode Column
HPLC Separation of Sodium and Four Inorganic Anions on Amaze TH Column
HPLC Analysis of 10 Water-Soluble Vitamins in HILIC, Cation- and Anion-Exchange Modes on Amaze TH Tri-Modal Column
HPLC Analysis of Polar Environmental Contaminants on Amaze TH Mixed-Mode Column with LC/MS Compatible Conditions
Separation of Phosphate, Thiophosphate and Sodium Ions on Amaze TH Column
HPLC Analysis of Trifluoroacetic Acid (TFA) on Amaze TH Mixed-Mode Column
HPLC Analysis of Yellow 6 (Sunset Yellow) and Red 40 Dye (Allura Red) in Soft Drinks on Amaze TH Mixed-Mode Column
More Applications
Versatility:
Very good
Column/Stationary Phase Description:
Tri-modal HILIC mixed-mode stationary phase with HILIC, cation-exchange and anion-exchange properties. proprietary multi-functional zwitterionic ligand with weak acid and weak basic groups
Mechanisms of Interaction:
HILIC, cation-exchange, cation-exclusion and anion-exchange,
Class of Analytes:
Amino acids, organic and inorganic acids, basic and acidic drugs and corresponding counter-ions, environmental contaminants, sugars, structural isomers
Suggested Initial Mobile Phase Conditions and Buffers
70-80% ACN with 5-30 mM buffer pH 2-7. Buffers and additives: ammonium formate, ammonium acetate, phosphate buffers at low concentrations (5-10 mM), and organic or inorganic acids. AVOID alcohols in the mobile phase.
Method Development Notes:
Explore various amounts of acetonitrile, different pH and buffer concentrations for different selectivities. Watch for solubility of phosphate buffers in high organic. Observe the recommended pH for the column. make sure buffers and additives are compatible with the detection technique. Make sure you consider significant cation- and anion-exchange capacity when changing buffers and equilibrating the column. Anion-exchange properties exist within pH 2-7, and cation-exchange properties exist within pH 3.5-7. The column is compatible with 100% ACN and 100% water. The column can operate in pure HILIC, pure cation-exchange, cation-exclusion, and anion-exchange modes, and a combination of two or three modes
Application Examples:
HPLC Analysis of Eight Aminoglycosides on Amaze TCH Column with LC/MS Compatible Conditions
HPLC Analysis of Glyphosate and Ethephon on Amaze HA Mixed-Mode Column with LC/MS Compatible Conditions
HPLC Separation of Basic Quaternary and Acidic Herbicides in HILIC, Cation- and Anion-Exchange Modes on Amaze TCH
HPLC Separation of Monovalent Inorganic Cations and Anions in HILIC, Cation- and Anion-Exchange Modes on Amaze TCH
HPLC Separation of Dihydrostreptomycin and Streptomycin in HILIC, Cation-Exchange Modes on Amaze TCH Column
HPLC Separation of Leucine and Isoleucine in HILIC, Cation-Exchange Modes on Amaze TCH Column
More Applications
Versatility:
Excellent
Column/Stationary Phase Description:
Tri-modal HILIC mixed-mode stationary phase with HILIC, cation-exchange and anion-exchange properties. Acidic group pKa 4, basic group pKa 11.
Mechanisms of Interaction:
HILIC, cation-exchange, anion-exchange, cation-exclusion, and weakly chelating ligand.
Class of Analytes:
polar metabolites, amino acids, organic and inorganic acids, basic and acidic drugs and corresponding counter-ions, acidic drugs, environmental contaminants, sugars, and structural isomers.
Suggested Initial Mobile Phase Conditions and Buffers
80% to 50% ACN with 5-20 mM buffer pH 3-5.5, formic acid 0.1-0.5%, isocratic methods, single, double, and triple gradients. Buffers and additives: ammonium formate, ammonium acetate, phosphate buffers at low concentrations (5-10 mM), organic and inorganic acids. AVOID alcohols in the mobile phase.
Method Development Notes:
Explore variations of acetonitrile, different pH, and buffer concentration for different selectivities. Observe the recommended pH for the column. make sure buffers and additives are compatible with detection techniques. Make sure you consider significant cation- and anion-exchange capacity when changing buffers and equilibrating the column. Colum can operate in single or in mixed-mode. HILIC mode starts at 65-70% ACN, Anion-exchange properties exist within pH 2-7, and cation-exchange properties exist within pH 3-7. The column is compatible with 100% ACN and 100% water. The column can operate in pure HILIC, pure cation-exchange, cation-exclusion, and anion-exchange modes, and a combination of two or three modes
Applications:
New Applications Coming Soon
Versatility:
Very good
Column/Stationary Phase Description:
HILIC mixed-mode stationary phase, weak RP, anion-exchange, and cation-exclusion, Low molecular weight polymer with embedded polar and ionizable basic groups. The basic group pKa is 10.
Mechanisms of Interaction:
Weak HILIC, weak reversed-phase, anion-exchange, and cation-exclusion.
Class of Analytes:
organic and inorganic acids, highly hydrophobic analytes, and structural isomers.
Suggested Initial Mobile Phase Conditions and Buffers
5-30% ACN with 10-30 mM buffer pH 2-7 for RP, 75-80% ACN with 10-30 mM buffer pH 2-7 for HILIC. Buffers and additives: ammonium formate, ammonium acetate, phosphate buffers, organic and inorganic acids
Method Development Notes:
Explore various amounts of acetonitrile, different pH and buffer concentrations for different selectivities. Watch for solubility of phosphate buffers in high organic. Observe the recommended pH for the column. make sure buffers and additives are compatible with detection techniques. Make sure you consider significant anion-exchange capacity when changing buffers and equilibrating the column. The column has weak HILIC and weak RP properties, anion-exchange operational range is pH 2-7. The column is compatible with 100% ACN and 100% water. The column can operate in pure reversed-phase, HILIC, cation-exclusion, or anion-exchange modes, and a combination of two or three modes
Application Examples:
Quantitative UV HPLC Analysis of Trifluoroacetic Acid in Peptide/Protein on Amaze HA Column
Quantitative UV HPLC Analysis of Vitamin C in Juices on Amaze HA Column
Quantitative Analysis of Phosphoric Acid in Soft Drinks on Amaze HA Column
HPLC Separation of Isomers of Ascorbic Acid in Reversed-Phase and Anion-Exchange Modes on Amaze HA Column
HPLC Separation of Acetic and Formic Acids on Amaze HA Mixed-Mode Column
HPLC Separation of 15 Organic Acids on Amaze HA Mixed-Mode Column with a Triple Gradient
HPLC Analysis of Uridine-Based Nucleotides on Amaze HA Mixed-Mode Column
Direct HPLC Analysis of Acidic Drug Fosfomycin in Human Blood on Amaze HA Mixed-Mode Column
More Applications
Reversed-Phase Mixed-Mode Columns
Versatility:
Good
Column/Stationary Phase Description:
Bi-modal reversed-phase mixed-mode stationary phase with reversed-phase, strong cation-exchange, and moderate anion-exclusion properties. Hydrophobic chain with acidic embedded groups, placed close to the surface of silica gel. Pka of the acidic group is 0.
Mechanisms of Interaction:
Reversed-phase, cation-exchange, and anion-exclusion.
.
Class of Analytes:
Amino acids, hydrophilic neutral and hydrophobic acidic compounds, inorganic cations, hydrophilic basic compounds, basic drugs, hydrophobic anionic counter-ions, and structural isomers.
Suggested Initial Mobile Phase Conditions and Buffers
10-70% ACN with 0.1-0.3% of organic or inorganic acid or 10-50 mM buffer. Buffers and additives: ammonium formate, ammonium acetate, phosphate buffers, and organic and inorganic acids. AVOID alcohols in the mobile phase.
Method Development Notes:
Explore variations of acetonitrile, buffer pH buffer concentration, and buffer nature for enhanced selectivities of separation for a broad range of compounds. Make sure you consider significant cation-exchange capacity when changing buffers and equilibrating the column. Column can provide very strong cation-exchange interaction even at pH 2. High buffer concentration might be required for compounds with strong basic groups. The column is compatible with 100% ACN and 100% water. The column can operate in reversed-phase, pure cation-exchange, and anion-exclusion modes, and a combination of two or three modes
Applications:
New Applications Coming Soon
Versatility:
Very good
Column/Stationary Phase Description:
Bi-modal reversed-phase mixed-mode stationary phase with reversed-phase and cation-exchange. Hydrophobic chain with multiple polar embedded groups, the acidic groups placed in the middle and at the end of the ligand. The acidic group pKa is 1.5.
Mechanisms of Interaction:
Reversed-phase, cation-exchange, and anion-exclusion.
Class of Analytes:
Amino acids, hydrophilic neutral and hydrophobic acidic compounds, inorganic cations, hydrophilic basic compounds, basic drugs and hydrophobic anionic counter-ions, structural isomers
Suggested Initial Mobile Phase Conditions and Buffers
10-70% ACN with 0.1-0.3% of organic or inorganic acid or 10-30 mM buffer pH 2-7. Buffers and additives: ammonium formate, ammonium acetate, phosphate buffers, and organic and inorganic acids. AVOID alcohols in the mobile phase.
Method Development Notes:
Explore variations of acetonitrile, buffer pH buffer concentration, and buffer nature for enhanced selectivities of separations for a broad range of compounds. Make sure you consider significant cation-exchange capacity when changing buffers and equilibrating the column. The column can operate in cation-exchange mode within pH 2-7. Stronger cation-exchange interactions can be observed at higher pH. The column is compatible with 100% ACN and 100% water. The column can operate in pure reversed-phase, pure cation-exchange, and anion-exclusion modes, and a combination of two or three modes.
Application Examples:
HPLC Analysis of Dopamine and 5 Amino Acids in Reversed-Phase and Cation-Exchange Modes on Amaze SC Column
HPLC Analysis of 20 Amino Acids on Amaze SC Mixed-Mode Column in RP and Cation-exchange Modes
HPLC Analysis of Acidic Drugs, Monovalent and Divalent Basic Counterions on Amaze SC Mixed-Mode Column
HPLC Analysis of Acidic Drug Ibuprofen and Basic Counterions on Amaze SC Mixed-Mode Column
HPLC Analysis of 4-Aminophenol in Acetaminophen Tablets on Amaze SC Mixed-Mode Column in Reversed-Phase and Cation-Exchange Modes
HPLC Separation of Alanine and Corresponding Esters on Amaze SC HPLC Column
HPLC Separation of Tricine and Tromethamine on Amaze SC Mixed-Mode Column
HPLC Separation of Hydrophilic Quaternary Amines (Betaine, Choline and Acethylcholine) on Amaze SC Mixed-Mode Column
More Applications
Versatility:
Very good
Column/Stationary Phase Description:
Bi-modal reversed-phase mixed-mode stationary phase with reversed-phase and cation-exchange. hydrophobic chain with polar embedded groups and acidic groups placed close to the surface of silica gel. Acidic group pKa 1.
Mechanisms of Interaction:
Reversed-phase, cation-exchange, and anion-exclusion.
Class of Analytes:
Amino acids, hydrophilic neutral and hydrophobic acidic compounds, inorganic cations, hydrophilic basic compounds, basic drugs and hydrophobic anionic counter-ions, structural isomers.
Suggested Initial Mobile Phase Conditions and Buffers
10-50% ACN with 0.03-0.2% of organic or inorganic acid or 10-50 mM buffer pH 2-7. Buffers and additives: ammonium formate, ammonium acetate, phosphate buffers, and organic and inorganic acids. AVOID alcohols in the mobile phase.
Method Development Notes:
Explore variations of acetonitrile, buffer pH buffer concentration, and buffer nature for enhanced selectivities of separations for a broad range of compounds. Make sure you consider significant cation-exchange capacity when changing buffers and equilibrating the column. The column is compatible with 100% ACN and 100% water. The column can operate in pure reversed-phase, pure cation-exchange, and anion-exclusion modes, and a combination of two or three modes. Carboxylic acid on the surface are ionized within the operational pH range and provide strong cation-exchange interaction.
Applications:
New Applications Coming Soon
Versatility:
Very good
Column/Stationary Phase Description:
Bi-modal reversed-phase mixed-mode stationary phase with reversed-phase and cation-exchange. hydrophobic chain with polar embedded groups and acidic groups placed close to the surface of silica gel. Acidic group pKa 2.
Mechanisms of Interaction:
Reversed-phase, cation-exchange, and anion-exclusion.
Class of Analytes:
Amino acids, hydrophilic neutral and hydrophobic acidic compounds, inorganic cations, hydrophilic basic compounds, basic drugs and hydrophobic anionic counter-ions, structural isomers.
Suggested Initial Mobile Phase Conditions and Buffers
10-50% ACN with 0.03-0.2% of organic or inorganic acid or 10-30 mM buffer pH 2-7. Buffers and additives: ammonium formate, ammonium acetate, phosphate buffers, and organic and inorganic acids. AVOID alcohols in the mobile phase.
Method Development Notes:
Explore variations of acetonitrile, buffer pH buffer concentration, and buffer nature for enhanced selectivity of separations for a broad range of compounds. Make sure you consider significant cation-exchange capacity when changing buffers and equilibrating the column. The column is compatible with 100% ACN and 100% water. The column can operate in pure reversed-phase, pure cation-exchange, and anion-exclusion modes, and a combination of two or three modes. Cation-exchange properties are partially suppressed at pH below 2.5.
Applications:
New Applications Coming Soon
Versatility:
Very good
Column/Stationary Phase Description:
Bi-modal reversed-phase mixed-mode stationary phase with reversed-phase and cation-exchange, weak chelating properties for primary amines and inorganic cations. hydrophobic chain with polar embedded groups, acidic group is placed close to the surface of silica gel, acidic group pKa is 3.
Mechanisms of Interaction:
Reversed-phase, cation-exchange, anion-exclusion, chelating.
Class of Analytes:
Amino acids, hydrophilic neutral and hydrophobic acidic compounds, inorganic cations, hydrophilic basic compounds, basic drugs and hydrophobic anionic counter-ions, structural isomers.
Suggested Initial Mobile Phase Conditions and Buffers
10-50% ACN with 0.1-0.3% of organic or inorganic acid or 20-30 mM buffer pH 3-7. Buffers and additives: ammonium formate, ammonium acetate, phosphate buffers, organic and inorganic acids. AVOID alcohols in the mobile phase.
Method Development Notes:
Explore variations of acetonitrile, buffer pH buffer concentration, and buffer nature for enhanced selectivities of separations for a broad range of compounds. Make sure you consider significant cation-exchange capacity when changing buffers and equilibrating the column. The column is compatible with 100% ACN and 100% water. The column can operate in pure reversed-phase, pure cation-exchange, and anion-exclusion modes, and a combination of two or three modes. Cation-exchange properties are partially suppressed at pH below 3.5.
Applications:
New Applications Coming Soon
Versatility:
Good
Column/Stationary Phase Description:
Reversed-phase mixed-mode stationary phase with reversed-phase, cation-exchange, and anion-exchange properties. Hydrophobic ligand with basic and acidic groups on the surface. The acidic group pKa is 1.5 and the basic group pKa is 10.
Mechanisms of Interaction:
Reversed-phase, cation-exchange, and anion-exchange.
Class of Analytes:
Basic and acidic drugs and corresponding counter-ions, organic and inorganic cations, and anions.
Suggested Initial Mobile Phase Conditions and Buffers
5-60% ACN with 10-30 mM buffer pH 2-7. Buffers and additives: ammonium formate, ammonium acetate, phosphate buffers, and organic and inorganic acids. AVOID alcohols in the mobile phase.
Method Development Notes:
Explore variations of acetonitrile, buffer pH buffer concentration, and buffer nature for enhanced selectivities of separations for a broad range of compounds. Make sure you consider significant cation- and anion-exchange capacity when changing buffers and equilibrating the column. Watch for solubility of phosphate buffers in high ACN mobile phases. The most versatile pH range of operation is 2-6. The column is compatible with 100% ACN and 100% water. The column can operate in pure reversed-phase, pure cation-exchange, and anion-exchange modes, and a combination of two or three modes. Cation-exchange properties are partially suppressed at pH below 2.5.
Applications:
New Applications Coming Soon
Versatility:
Good
Column/Stationary Phase Description:
Reversed-phase mixed-mode stationary phase with reversed-phase, cation-exchange, and anion-exchange properties. HILIC properties in high organic (ACN>70%). Hydrophobic polymer with polar embedded basic and acidic groups, acidic group pKa is 4, basic group pKa is 10.
Mechanisms of Interaction:
Reversed=[hase, HILIC, cation-exchange, anion-exchange, cation-exclusion.
Class of Analytes:
Basic and acidic drugs and corresponding counter-ions, environmental contaminants, organic and inorganic cations, and anions.
Suggested Initial Mobile Phase Conditions and Buffers
5-30% ACN with 10-30 mM buffer pH 3-7 for RP, and 75-80% ACN with 10-30 mM buffer pH 3-7 for HILIC. Buffers and additives: ammonium formate, ammonium acetate, phosphate buffers, and organic and inorganic acids. AVOID alcohols in the mobile phase
Method Development Notes:
Explore variations of acetonitrile, buffer pH buffer concentration, and buffer nature for enhanced selectivities of separations for a broad range of compounds. Make sure you consider significant cation- and anion-exchange capacity when changing buffers and equilibrating the column. Watch for solubility of phosphate buffers in high ACN mobile phases. The most versatile pH range of operation is 4-6. The column is compatible with 100% ACN and 100% water. The column can operate in pure reversed-phase, pure cation-exchange, cation-exclusion, and anion-exchange modes, and a combination of two or three modes. Cation-exchange properties are partially suppressed at pH below 4, and completely suppressed at pH below 2.5.
Application Examples:
HPLC Separation of Hydrophobic, Hydrophilic, Basic and Acidic Compounds on Amaze TR Mixed-Mode Column
HPLC Analysis of Dihydroxybenzoic Acids on Amaze TR Mixed-Mode Column
HPLC Analysis of Benzenesulfonic and p-Toluenesulfonic Acids on Amaze TR Mixed-Mode Column
HPLC Analysis of Aromatic Carboxylic Acids on Amaze TR Mixed-Mode Column
HPLC Separation of Glyphosate and Paraquat on Amaze TR Mixed-Mode Column
More Applications
Versatility:
Good
Column/Stationary Phase Description:
Reversed-phase, cation-exchange, and anion-exclusion stationary phase with weak cation-exchange group on the surface. hydrophobic chain with polar embedded groups with a weak cation-exchange group. The acidic group pKa is 5.
Mechanisms of Interaction:
Reversed-phase and cation-exchange.
Class of Analytes:
Hydrophilic and hydrophobic basic, hydrophobic acidic compounds, inorganic cations, basic drugs and anionic counter-ions, and structural isomers.
Suggested Initial Mobile Phase Conditions and Buffers
10-50% ACN with 0.05-0.2% of acid or 10-30 mM buffer pH 4-7. Buffers and additives: ammonium formate, ammonium acetate, phosphate buffers, and organic and inorganic acids. AVOID alcohols in the mobile phase.
Method Development Notes:
Explore variations of acetonitrile, buffer pH buffer concentration, and buffer nature for enhanced selectivities of separations for a broad range of compounds. Make sure you consider significant cation-exchange capacity when changing buffers and equilibrating the column. The column is compatible with 100% ACN and 100% water. The column can operate in pure reversed-phase, pure cation-exchange, and anion-exclusion modes, and a combination of two or three modes. Cation-exchange properties are partially suppressed at pH below 5, and completely suppressed below pH 3.5.
Application Examples:
HPLC Analysis of Drug Zantac 75 on Heritage MC Mixed-Mode Column
HPLC Analysis of Drug Loratadine and Related Impurities on Heritage MC Mixed-Mode Column
HPLC Analysis of DMF and N-Nitrosodimethylamine in Metformin Drug on Heritage MC Mixed-Mode Column
More Applications
Versatility:
Very good
Column/Stationary Phase Description:
Reversed-phase, anion-exchange, and cation-exclusion stationary phase with weak anion-exchange group on the surface. hydrophobic chain with weak basic polar embedded groups.
Mechanisms of Interaction:
Reversed phase, weak anion-exchange, cation-exclusion.
Class of Analytes:
Hydrophobic drugs and acidic counter-ions, hydrophobic neutral and hydrophobic weakly acidic compounds, basic polymers, strong inorganic and organic acids.
Suggested Initial Mobile Phase Conditions and Buffers
10-50% ACN with 0.05-0.2% of acid or 10-30 mM buffer pH 2-7. Buffers and additives: ammonium formate, ammonium acetate, phosphate buffers, and organic and inorganic acids.
Method Development Notes:
Explore variations of acetonitrile, buffer pH buffer concentration, and buffer nature for enhanced selectivities of separations for a broad range of compounds. Make sure you consider significant cation-exchange capacity when changing buffers and equilibrating the column. The column is compatible with 100% ACN and 100% water. The column can operate in pure reversed-phase, pure cation-exclusion, and anion-exchange modes, and a combination of two or three modes. Weak anion-exchange properties within pH 2-7.
Application Examples:
New Applications Coming Soon
Versatility:
Good
Column/Stationary Phase Description:
Reversed-phase, cation-exchange, and anion-exclusion stationary phase with intermediate/weak anion-exchange group on the surface. hydrophobic chain with intermediate/weak basic polar embedded groups. The basic group pKa is 9.
Mechanisms of Interaction:
Reversed-phase, intermediate anion-exchange, and cation-exclusion.
Class of Analytes:
Hydrophobic basic drugs and acidic counter-ions, hydrophobic neutral and hydrophobic weakly acidic compounds, basic polymers, strong and weak inorganic and organic acids.
Suggested Initial Mobile Phase Conditions and Buffers
10-50% ACN with 0.05-0.2% of acid or 10-50 mM buffer pH 2-7. Buffers and additives: ammonium formate, ammonium acetate, phosphate buffers, and organic and inorganic acids.
Method Development Notes:
Explore variations of acetonitrile, buffer pH buffer concentration, and buffer nature for enhanced selectivities of separations for a broad range of compounds. Make sure you consider significant cation-exchange capacity when changing buffers and equilibrating the column. The column is compatible with 100% ACN and 100% water. The column can operate in pure reversed-phase, pure cation-exclusion, and anion-exchange modes, and a combination of two or three modes. Intermediate strength anion-exchange properties within pH 2-7.
Application Examples:
New Applications Coming Soon
Versatility:
Good
Column/Stationary Phase Description:
Reversed-phase mixed-mode stationary phase, anion-exchange and cation-exclusion properties. Hydrophobic ligand with a basic group close to the surface of silica gel. The basic group pKa is 10.
Mechanisms of Interaction:
Reversed-phase, intermediate anion-exchange, and cation-exclusion.
Class of Analytes:
Hydrophobic basic drugs and acidic counter-ions, hydrophobic neutral and hydrophobic weakly acidic compounds, basic polymers, strong and weak inorganic and organic acids.
Suggested Initial Mobile Phase Conditions and Buffers
10-50% ACN with 0.05-0.2% of acid or 10-50 mM buffer pH 2-7. Buffers and additives: ammonium formate, ammonium acetate, phosphate buffers, and organic and inorganic acids.
Method Development Notes:
Explore variations of acetonitrile, buffer pH buffer concentration, and buffer nature for enhanced selectivities of separations for a broad range of compounds. Make sure you consider significant cation-exchange capacity when changing buffers and equilibrating the column. The column is compatible with 100% ACN and 100% water. The column can operate in pure reversed-phase, pure cation-exclusion, and anion-exchange modes, and a combination of two or three modes. Intermediate strength anion-exchange properties within pH 2-7.
Applications:
New Applications Coming Soon
Versatility:
Very good
Column/Stationary Phase Description:
Reversed-phase, anion-exchange, and cation-exclusion stationary phase with a strong anion-exchange group on the surface.
Mechanisms of Interaction:
Reversed-phase, anion-exchange, and cation-exclusion.
Class of Analytes:
Hydrophobic basic drugs and acidic counter-ions, hydrophobic neutral and hydrophobic weakly acidic compounds, basic polymers, and inorganic/organic acids.
Suggested Initial Mobile Phase Conditions and Buffers
30-60% ACN with 0.1-0.3% of acid or 20-100 mM buffer ph 2-5. Buffers and additives: ammonium formate, ammonium acetate, phosphate buffers, and organic and inorganic acids.
Method Development Notes:
Explore variations of acetonitrile, buffer pH buffer concentration, and buffer nature for enhanced selectivities of separations for a broad range of compounds. Make sure you consider significant anion-exchange capacity when changing buffers and equilibrating the column. The column is compatible with 100% ACN and 100% water. The column can operate in pure reversed-phase, pure cation-exclusion, and anion-exchange modes, and a combination of two or three modes. Strong to medium anion-exchange properties within pH 2-7.
Application Examples:
HPLC Analysis of Five Acidic Drugs on Heritage MA Mixed-Mode Column
HPLC UV Analysis of Four Basic Drugs and Six Acidic Counterions on Heritage MA Mixed-Mode Column
HPLC Analysis of Ascorbic, Citric and Ethylenediaminetetraacetic acids on Heritage MA Mixed-Mode Column
HPLC UV Analysis of Polyethyleneimine on Heritage MA Column in HPLC Analysis of Parabens on Heritage MA Mixed-Mode Column
Ion-Exclusion and Size-Exclusion Modes
HPLC Analysis of Seven Anions on Heritage MA Mixed-Mode Column
HPLC Analysis of Nitrite and Nitrate Ions on Heritage MA Mixed-Mode Column
Simultaneous HPLC Analysis of Brompheniramine and Maleic Acid Counter-Ion on Heritage MA Column
HPLC Analysis of Six Organic Acids on Heritage MA Mixed-Mode Column
More Applications
Core-Shell Mixed-Mode Columns
Versatility:
Excellent
Column/Stationary Phase Description:
Reversed-phase, cation-exchange, and anion-exclusion stationary phase with weak cation-exchange group in the surface. Combination of high-efficiency core-shell silica gel and unique selectivity of mixed-mode columns. hydrophobic chain with an acidic group close to the surface of core-shell silica gel. Acidic pKa is 2.
Mechanisms of Interaction:
Reversed-phase, weak cation-exchange, and anion-exclusion.
Class of Analytes:
Amino acids, hydrophilic neutral and hydrophobic acidic compounds, inorganic cations, hydrophilic basic compounds, basic drugs, anionic counter-ions, and structural isomers.
Suggested Initial Mobile Phase Conditions and Buffers
10-50% ACN with 0.05-0.2% of acid or 10-30 mM buffer pH 2-7. Buffers and additives: ammonium formate, ammonium acetate, phosphate buffers, and organic and inorganic acids.AVOID alcohols in the mobile phase.
Method Development Notes:
Explore variations of acetonitrile, buffer pH buffer concentration, and buffer nature for enhanced selectivities of separations for a broad range of compounds. Make sure you consider significant cation-exchange capacity when changing buffers and equilibrating the column. The column is compatible with 100% ACN and 100% water. The column can operate in pure reversed-phase, pure cation-exchange, and anion-exclusion modes, and a combination of two or three modes. Cation-exchange properties are partially suppressed at pH below 2.5.
Application Examples:
Versatility:
Excellent
Column/Stationary Phase Description:
This stationary phase was designed for the analysis of polar metabolites with LC/MS/MS. Reversed-phase, cation-exchange, and anion-exclusion stationary phase with weak cation-exchange group in the surface. Combination of high-efficiency core-shell silica gel and unique selectivity of mixed-mode columns. hydrophobic chain with an acidic group close to the surface of core-shell silica gel. Acidic pKa is 2.
Mechanisms of Interaction:
Well-balanced reversed-phase and cation-exchange.
Class of Analytes:
Polar metabolites, amino acids, polar amines, hydrophilic neutral and hydrophobic acidic compounds, and hydrophilic basic compounds.
Suggested Initial Mobile Phase Conditions and Buffers
5-50% ACN with 0.05-0.2% of acid or 5-20 mM buffer pH 2-7. Buffers and additives: ammonium formate, ammonium acetate, phosphate buffers, and organic and inorganic acids. AVOID alcohols in the mobile phase.
Method Development Notes:
Explore variations of acetonitrile, buffer pH buffer concentration, and buffer nature for enhanced selectivities of separations for a broad range of compounds. Make sure you consider significant cation-exchange capacity when changing buffers and equilibrating the column. The column is compatible with 100% ACN and 100% water. The column can operate in pure reversed-phase, pure cation-exchange, and anion-exclusion modes, and a combination of two or three modes. Cation-exchange properties are partially suppressed at pH below 2.5.
Application Examples:
New Applications Coming Soon
Versatility:
Very good
Column/Stationary Phase Description:
Reversed-phase, anion-exchange, and cation-exclusion stationary phase with the strong anion-exchange group on the surface. Combination of high-efficiency of core-shell silica gel and unique selectivity of mixed-mode columns, hydrophobic chain with polar ionizable groups. The basic group pKa is 14.
Mechanisms of Interaction:
Reversed-phase, strong anion-exchange, cation-exclusion, size-exclusion for basic polymer.
Class of Analytes:
Hydrophobic drugs and acidic counter-ions, hydrophobic neutral and hydrophobic weakly acidic compounds, basic polymers, and inorganic and organic acids.
Suggested Initial Mobile Phase Conditions and Buffers
5-50% ACN with 10-100 mM buffer pH 2-5 or 0.05-0.2% acid, ammonium formate. Buffers and additives: ammonium formate, ammonium acetate, phosphate buffers, and organic and inorganic acids.
Method Development Notes:
Explore variations of different amounts of ACN, buffer concentration, and pH. Watch for solubility of phosphate buffers in high organic. Observe the recommended pH for the column. make sure buffers and additives are compatible with the detection technique. Make sure you consider moderate cation-exchange capacity when changing buffers and equilibrating the column. The column is compatible with 100% ACN and 100% water. The column can operate in pure reversed-phase, pure cation-exclusion, and anion-exchange modes, and a combination of two or three modes. Strong to medium anion-exchange properties within pH 2-7.
Application Examples:
Versatility:
Good
Column/Stationary Phase Description:
HILIC mixed-mode stationary phase with HILIC, cation-exchange and anion-exclusion properties. Combination of high efficiency of core-shell particles and unique selectivity of mixed-mode ligands. Hydrophilic short acidic chain, pKa of acid 2.5.
Mechanisms of Interaction:
HILIC, cation-exchange, and anion-exclusion.
Class of Analytes:
amino acids, organic and inorganic bases, organic and inorganic acids, basic drugs, acidic drugs, environmental contaminants, sugars, and structural isomers.
Suggested Initial Mobile Phase Conditions and Buffers
60-80% ACN with 10-30 mM buffer pH 2-7.Buffers and additives: ammonium formate, ammonium acetate, phosphate buffers, and organic and inorganic acids.
Method Development Notes:
Explore variations of different amounts of ACN, buffer concentration, and pH. Watch for solubility of phosphate buffers in high organic. Observe the recommended pH for the column. make sure buffers and additives are compatible with the detection technique. Make sure you consider moderate cation-exchange capacity when changing buffers and equilibrating the column. The column is compatible with 100% ACN and 100% water. The column can operate in pure HILIC, pure cation-exchange, and anion-exclusion modes, and a combination of two or three modes.
Application Examples:
Fast HPLC Separation of Uracil and Uridine on Core-Shell Mixed-Mode Coresep S Column in HILIC Mode
HPLC Analysis of Underivatized Amino Acids on Coresep S Column in HILIC and Cation-Exchange Modes
More Applications
Versatility:
Good
Column/Stationary Phase Description:
Proprietary HILIC/anion-exclusion stationary phase designed for analysis of polar compounds in HILIC mixed-mode
Mechanisms of Interaction:
HILIC, unspecified ionic interactions.
Class of Analytes:
Oligonucleotides, sugars amino acids
Suggested Initial Mobile Phase Conditions and Buffers
50-70% ACN with 5-30 mM buffer ph 2-7. Buffers and additives: ammonium formate, ammonium acetate, phosphate buffers, and organic and inorganic acids.
Method Development Notes:
Explore variations of different amounts of ACN, buffer concentration, and pH. Watch for solubility of phosphate buffers in high organic. Observe the recommended pH for the column. make sure buffers and additives are compatible with the detection technique. Make sure you consider moderate cation-exchange capacity when changing buffers and equilibrating the column. The column is compatible with 100% ACN and 100% water. The column can operate in pure HILIC, pure cation-exchange, and anion-exclusion modes, and a combination of two or three modes.
Application Examples:
Reversed-Phase and Aromatic Columns
Versatility:
Good
Column/Stationary Phase Description:
Reversed-phase stationary phase with reversed-phase properties. Polar-embedded groups, no ionizable groups
Mechanisms of Interaction:
Reversed-phase
Class of Analytes:
Hydrophobic neutral, hydrophobic acidic and hydrophobic basic compounds, structural isomers, cannabinoids, steroids, terpenes, various phenols
Suggested Initial Mobile Phase Conditions and Buffers
10-60% ACN or MeOH with 0.1-0.3% of acid or 10-20 mM buffer ph 2-7. No buffers or additives, ammonium formate, ammonium acetate, phosphate buffers, and organic and inorganic acids.
Method Development Notes:
Explore variation of ACN and methanol as well as additives for alternative selectivities of separation. The column provides alternative selectivity to other manufacturers. Column is compatible with 100% organic and 100% water.
Application Examples:
More Applications
Versatility:
Very good
Column/Stationary Phase Description:
Reversed-phase stationary phase with reversed-phase properties and additional pi-pi interactions. proprietary C18-Phenyl ligand. Polar-embedded groups, no ionizable groups
Mechanisms of Interaction:
Reversed-phase, dipole-dipole, pi-pi
Class of Analytes:
Hydrophobic neutral, hydrophobic acidic and hydrophobic basic compounds, structural isomers, cannabinoids, steroids, terpenes, various phenols
Suggested Initial Mobile Phase Conditions and Buffers
10-60% ACN or MeOH with 0.1-0.3% of acid or 10-20 mM buffer ph 2-7. No buffers or additives, ammonium formate, ammonium acetate, phosphate buffers, and organic and inorganic acids. Use methanol for enhanced pi-pi interactions.
Method Development Notes:
Explore variation of ACN and methanol as well as additives for alternative selectivities of separation. The column provides alternative selectivity to other manufacturers. Column is compatible with 100% organic and 100% water.
Application Examples:
Versatility:
Very good
Column/Stationary Phase Description:
Polyfluorinated stationary phase with reversed-phase and pi-pi properties. proprietary C18-Polyfluorinated aromatic group. Polar-embedded groups, no ionizable groups
Mechanisms of Interaction:
Reversed-phase, dipole-dipole, pi-pi.
Class of Analytes:
Hydrophobic neutral, hydrophobic acidic, and hydrophobic basic compounds, structural isomers, cannabinoids, steroids, terpenes, various phenols
Suggested Initial Mobile Phase Conditions and Buffers
10-60% ACN or MeOH with 0.1-0.3% of acid or 10-20 mM buffer ph 2-7. No buffers or additives, ammonium formate, ammonium acetate, phosphate buffers, and organic and inorganic acids. Use methanol for enhanced pi-pi interactions.
Method Development Notes:
Explore variations of ACN and methanol as well as additives for alternative selectivities of separation. The column provides unique selectivity for the separation of hydrophobic and aromatic compounds. The column provides alternative selectivity to other manufacturers. Column is compatible with 100% organic and 100% water.
Application Examples:
HPLC Analysis of 6 Aromatic Alcohols, Acids and Neutral Compounds on Amaze Aromatic Stationary Phases.
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Versatility:
Excellent
Column/Stationary Phase Description:
Polyfluorinated stationary phase with two aromatic groups for enhanced selectivity of separation. Polar-embedded groups, no ionizable groups
Mechanisms of Interaction:
Reversed-phase, dipole-dipole, pi-pi. Unique selectivity due to proprietary ligand design. Unique and enhanced pi-pi interactions.
Class of Analytes:
Hydrophobic neutral, hydrophobic acidic, and hydrophobic basic compounds, structural isomers, cannabinoids, steroids, terpenes, various phenols
Suggested Initial Mobile Phase Conditions and Buffers
10-60% ACN or MeOH with 0.1-0.3% of acid or 10-20 mM buffer ph 2-7, no buffers or additives, ammonium formate, ammonium acetate, phosphate buffers, organic and inorganic acids
Method Development Notes:
Explore variations of ACN and methanol as well as additives for alternative selectivities of separation. Two aromatic fluorinated rings provide unique selectivity for separation and enhanced resolution between analytes. Recommended for analysis of close-eluting aromatic compounds. The column provides alternative selectivity to other manufacturers. Column is compatible with 100% organic and 100% water.
Application Examples:
Selectivity Comparison for Amaze SPF Aromatic Phase with Methanol vs. Acetonitrile
Selectivity Comparison for Amaze AR Aromatic Phase with Methanol vs. Acetonitrile
Selectivity Comparison for Three Amaze Aromatic Stationary Phases with MeOH/Water
Selectivity Comparison for Three Amaze Aromatic Stationary Phases with ACN/Water
HPLC Analysis of 6 Aromatic Acids and Alcohols on Amaze Aromatic Stationary Phases
HPLC Analysis of 6 Aromatic Acidic, Zwitterionic and Neutral Compounds on Amaze Aromatic Stationary Phases
HPLC Analysis of 6 Aromatic Alcohols, Acids and Neutral Compounds on Amaze Aromatic Stationary Phases.
More Applications
