HPLC Separation of Acetic and Formic Acids on Amaze HA Mixed-Mode Column

Conditions of Experiment
Column: Amaze HA
Separation Modes: reversed-phase, anion-exchange
Column Dimenstions: 4.6x100 mm, 3 um, 100a
Mobile Phase: ACN/water/buffer, ACN/water/accid
Detection: 205 nm
Sample: 0.5 mg/ml
Injection: 2 uL
Flow rate: 1 ml/min
Class of compounds: Organic acid
Nature of compounds: Acidic
Compounds: Acetic acid, Formic acid