HPLC Method Development

HELIX Chromatography provides several options for Method Development Services. Experience scientists will be assigned to the project to develop your methods. We will sign a Non-Disclosure Agreement if your company requires this. Once we have details for your separation we can estimated the time required for the method and let you know when we can start on your project. You have the right to reject our recommendation and decide on your own what service to use. Once we develop a method we will ask you to review it and let us know if anything else needs to be done. We will use our columns to develop a method, but there is no obligation to buy any columns.

We try to start method development within two days of receiving samples, but we can also accommodate rush requests. If we have the required compounds in our library we can use them to develop a method for you, otherwise you’ll need to provide samples and standards. Providing us with samples that are not in solution can help us develop method much faster. We require 5-10 mg of each standard/marker. We require submission of standards or markers to identify peaks in your specific mixture.

We provide various tiers of service:

  1. Basic Free MDS. The basic free screening method development includes screening done with 2 columns and 2 different mobile phase. Total time allowance will not exceed 3 hours, and will be scheduled as time permits. Chromatograms and raw data may be provided if needed. We reserve the right to terminate the project if the time is exceeded, or ask customer to move to a paid tier. Customer is updated when project is completed.
  2. Silver MDS. Paid Method Development Services. Screening as many as 5 columns with 3 mobile phases. Total time allowance is 8 hours at $125/h. Daily updates on the project upon request. After 8 hours the customer has a choice to terminate the project or move to the next tier option. Providing chromatograms in PowerPoint/PDF format, and raw data per request.
  3. Gold MDS. Paid Extended Method Development Services. Includes finding optimum conditions achieving desired and specified resolution between compounds. Time allowance is up to 40 hours at $125/h. After 40 hours customer has a choice to terminate the project or move to the next tier option. Daily updates on the project upon request. Providing report, chromatograms, raw data and recommendations.
  4. Diamond MDS. Pre-Validation Method Development Services. Includes finding optimum conditions, achieving desired and specified resolution between compounds. Testing 3 different lots of columns to provide reproducibility data. Conducting quantitation and linearity studies. Time allowance varies on the project. Daily updates on the project upon request. Providing report, chromatograms, raw data and recommendations. 30% discount for the first 3 columns upon receiving the payment for method development

 

Method development examples

HPLC Analysis of Hemp Oil and CBD Isolate for CBD and THC ContentHPLC Analysis of Hemp Oil and CBD Isolate for CBD and THC Content
BD Distillate is a distilled extract derived from hemp biomass (flower and plant). CBD distillate usually contains 80%+ of CBD with the rest being other minor cannabinoids, terpenes, and other plants oils and extracts. The federal regulations call for tetrahydrocannabinol to be less than 0.3%. We have developed an HPLC procedure for analysis of CBD and THC in hemp products. This method can be applied to analysis of all cannabinoids found in hemp and marijuana plants. The same methodology can be used for your cannabis analysis.

Request more information about analysis of hemp oil and CBD isolate for CBD and THC content.

 

 

 

HPLC Analysis N-Nitrosodiethylamine in Valsartan Drug FormulationHPLC Analysis N-Nitrosodiethylamine in Valsartan Drug Formulation
In recent months several blood pressure medications were recalled for the presence of potentially cancerogenic impurity – N-Nitrosodiethylamine. We have developed a procedure for quantitative analysis of N-nitrosamines in blood pressure formulations. You can use our columns and method for analysis of impurities and API in drugs and drug formulations.

Request more information about analysis of n-nitrosodiethylamine in valsartan drug formulation.

 

 

 

 

HPLC Analysis of Trace Levels of Paraquat and Glyphosate in Fruits and VegetablesHPLC Analysis of Trace Levels of Paraquat and Glyphosate in Fruits and Vegetables
Paraquat and glyphosate are two of the most widely used herbicides world wide. Both compounds are very polar, ionic, with an opposite charge. Paraquat is a hydrophilic highly basic compound and glyphosate is polar acidic compound. We have developed stationary phase and method for simultaneous analysis of these two herbicides in various matrices, including fruits, vegetables, drinking and waste waters. The approach and column are suitable for analysis of glyphosate, paraquat and other herbicides/pesticides in different samples.

Request more information about analysis of trace levels of paraquat and glyphosate in fruits and vegetables.

 

 

 

HPLC Analysis of Process Waste WaterHPLC Analysis of Process Waste Water in Biodiesel Production. Part 1. Phosphate Ion and Total Organic Analysis
We have developed a new approach to analysis of total organic matter and phosphate ion in the wastewater in biodiesel production. A short 3-minute method allows to quantify organic components and phosphate ion levels in wastewater streams. The method is robust and LC/MS compatible. Amaze N1 mixed-mode column was used to retain and separate target compounds.

Request more information about analysis of process waste water in biodiesel production.

 

 

 

 

HPLC Analysis of Filter Deposits from Biodiesel Production HPLC Analysis of Filter Deposits from Biodiesel Production
Biodiesels are produced in large quantities using transesterification of animal and vegetable fats/oils. Optimization of the manufacturing cost is directly related to successful and economically viable use of biodiesel. One of the bottlenecks of the production is the issue with filtration and buildup on the filter during the production, which significantly slows down the entire filtration process. Up to now, there has been no study on what causes the drastic slowdown of filtration during biodiesel production. We have conducted such a study on the filter deposits and were able to develop a group analysis for components of biodiesel adsorbed on fiberglass filters. Active measures to minimize these deposits were implemented, increasing the rate of filtration. Same methodology can be used for analysis of your biodiesel production. A selective mixed-mode HPLC method for analysis of glycerides, triglycerides, phospholipids and fatty acids present in raw material was developed.

Request more information about analysis of biodiesel, glycerides, triglycerides, phospholipids and fatty acids.