HPLC Separation of 15 Organic Acids on Amaze HA Mixed-Mode Column with a Triple Gradient

Conditions of Experiment
Column: Amaze HA
Separation Modes: reveresed-phase , anion-exchange
Column Dimenstions: 4.6x100, 3 um, 100A
Mobile Phase: "A: 2% ACN with 40 mM ammonium phosphate pH 4.0 B: 20% ACN with 100 mM ammonium phosphate pH 2.0 gradient from 100% A to 100% B in 15 minutes, 5 minutes hold "
Detection: 205 nm
Sample: 0.2-0.5 mg/ml
Injection: 2 uL
Flow rate: 1 ml/min
Class of compounds: Aromatic acid, Isomer, Organic acid, Organic compound, Preservative, Supplement, Vitamin
Nature of compounds: Acidic, Hydrophilic, Hydrophobic, Neutral, Polar
Compounds: Acetic acid, Ascorbic acid, Formic acid, Succinic acid, Citric acid, Malonic acid, Fumaric acid, Mandelic acid, Oxalic acid, Terephthalic acid, Benzoic acid, Maleic acid, Phthalic acid, Benzenesulfonic acid, p-Toluenesulfonic acid