HPLC Analysis of 4-Aminophenol in Acetaminophen Tablets on Amaze SC Mixed-Mode Column in Reversed-Phase and Cation-Exchange Modes

Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase cation-exchange
Column Dimenstions: 3.0x100 mm, 5 μm, 100A
Mobile Phase: 2% ACN wiith 50 mM AmFm pH 3
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Class of compounds: Amines, Aromatic base, Aromatic compound, Drug
Nature of compounds: Basic, Hydrophilic, Neutral, Polar
Compounds: Acetaminophen, 4-Aminophenol