HPLC Separation of Hydrophobic, Hydrophilic, Basic and Acidic Compounds on Amaze TR Mixed-Mode Column
Conditions of Experiment
Column: | Amaze TR |
Separation Modes: | RP, cation-exchange, anion-exchange |
Column Dimenstions: | 4.6x150 mm, 5 um, 100A |
Mobile Phase: | 30% ACN with 30 mM AmAc pH 5 |
Detection: | UV 275 nm |
Sample: | 0.2-0.3 mg/ml |
Injection: | 3 uL |
Flow rate: | 1 ml/min |
Analytes
Class of compounds: | Aromatic base, Aromatic compound, Catecholamine, Drug, Neurotransmitter, Organic acid, Organic compound, Preservative, Stimulant |
Nature of compounds: | Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar |
Compounds: | Norphenylephrine, Ephedrine, 3,4-Dihydroxybenzoic acid, Benzenesulfonic acid, Propylparaben, Propranolol |