HPLC Analysis of Nitrite and Nitrate Ions on Heritage MA Mixed-Mode Column

Conditions of Experiment
Column: Heritage MA
Separation Modes: anion-exchange
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: 60% ACN with 60 mM AmFm pH 3
Detection: UV 255 nm
Sample: 0.5 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Inorganic anion, Organic acid
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Nitrite ion, Nitrate ion