HPLC Analysis of Nitrite and Nitrate Ions on Heritage MA Mixed-Mode Column

Application description

Nitrates and nitrites are polar inorganic ions. Nitrates are mainly used as fertilizers and as the oxidizing agents in the production of explosives. Both nitrates and nitrites are used as food additives/components. Both ions are considered toxic and methods for determination in various samples are very critical for examples in food safety. We have developed a simple mixed-mode approach to separate and analyze these two ions in one run. Both ions have good UV activity and a UV detector can be used for monitoring these two ions during the elution from the Heritage MA mixed-mode HPLC column. This method is also compatible with MS, ELSD and CAD detection, and can be used for analysis of acidic organic and inorganic ions in one run. The retention time is adjusted by the buffer pH and buffer concentration.

Conditions of Experiment
Column: Heritage MA
Separation Modes: anion-exchange
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: 60% ACN with 60 mM AmFm pH 3
Detection: UV 255 nm
Sample: 0.5 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Inorganic anion, Organic acid
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Nitrite ion, Nitrate ion