Fast HPLC Separation of Uracil and Uridine on Core-Shell Mixed-Mode Coresep S Column in HILIC Mode
Application description
Uracil and Uridine are nucleosides which occur as a part of RNA. Both compounds are neutral in nature and very polar. Uracil is often used as a void marker in reversed-phase HPLC. A fast HILIC-based method was developed for analysis of uracil and uridine on the core-shell HILIC and cation-exchange column. Method provides good peak shape and retention control. Column and method can be used for analysis of polar compounds in HILIC, and HILIC/mixed-mode.
Conditions of Experiment
Column: | Coresep S |
Separation Modes: | HILIC |
Column Dimenstions: | 3.2 x 100 mm, 2.7 um, 90A |
Mobile Phase: | 98% ACN with no additives |
Detection: | UV 250 nm |
Sample: | 0.1-0.3 mg/ml |
Injection: | 1 uL |
Flow rate: | 0.5 mg/ml |
Analytes
Class of compounds: | Nucleobase, Nucleoside |
Nature of compounds: | Hydrophilic, Neutral |
Compounds: | Uracil, Uridine |