Fast HPLC Separation of Uracil and Uridine on Core-Shell Mixed-Mode Coresep S Column in HILIC Mode

Application description

Uracil and Uridine are nucleosides which occur as a part of RNA. Both compounds are neutral in nature and very polar. Uracil is often used as a void marker in reversed-phase HPLC. A fast HILIC-based method was developed for analysis of uracil and uridine on the core-shell HILIC and cation-exchange column. Method provides good peak shape and retention control. Column and method can be used for analysis of polar compounds in HILIC, and HILIC/mixed-mode.

Conditions of Experiment
Column: Coresep S
Separation Modes: HILIC
Column Dimenstions: 3.2 x 100 mm, 2.7 um, 90A
Mobile Phase: 98% ACN with no additives
Detection: UV 250 nm
Sample: 0.1-0.3 mg/ml
Injection: 1 uL
Flow rate: 0.5 mg/ml
Class of compounds: Nucleobase, Nucleoside
Nature of compounds: Hydrophilic, Neutral
Compounds: Uracil, Uridine