HPLC Separation of Glyphosate and Paraquat on Amaze TR Mixed-Mode Column

Application description

Paraquat and glyphosate are two of the most widely used herbicides worldwide. Both compounds are very pola and ionic, with an opposite charge. Paraquat is a hydrophilic, highly basic compound and glyphosate is polar acidic compound. We have developed a stationary phase and method for the simultaneous analysis of these two herbicides in various matrices, including fruits, vegetables, drinking and waste waters. The approach and column are suitable for the analysis of glyphosate, paraquat and other herbicides/pesticides in different samples. The retention time, selectivity and resolution can be adjusted with the amount of acetonitrile, buffer and buffer pH. Buffer pH controls the ionization state of both the stationary phase and analytes, thus affecting the ionic mechanism of interaction in addition to hydrophobic or reversed-phase interactions. The column is suitable for the analysis of hydrophobic, hydrophilic, basic, acidic and neutral herbicides and pesticides. The method is compatible with ELSD, CAD and mass spectrometry. Glyphosate has very limited UV activity and needs an alternative to the UV detection technique. For most of the complex matrices like plants, fruits, vegetables, grains, soil, and waste waters, some sample preparation with SPE or QuEChERS is required.

Conditions of Experiment
Column: Amaze TR
Separation Modes: reversed-phase, cation-exchange, anion-exchange
Column Dimenstions: 4.6x50 mm, 5 um, 100A
Mobile Phase: ACN from 90% to 30%, AmFm pH 3 from 10 mM to 40 mM in 7 minutes
Detection: UV 275 nm, ELSD
Sample: 0.3 mg/ml
Injection: 5 uL
Flow rate: 1 ml/min
Class of compounds: Amines, Herbicide, Organic acid, Pesticide
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Glyphosate, Paraquat