HPLC Separation of Glyphosate and Paraquat on Amaze TR Mixed-Mode Column

Conditions of Experiment
Column: Amaze TR
Separation Modes: reversed-phase, cation-exchange, anion-exchange
Column Dimenstions: 4.6x50 mm, 5 um, 100A
Mobile Phase: ACN from 90% to 30%, AmFm pH 3 from 10 mM to 40 mM in 7 minutes
Detection: UV 275 nm, ELSD
Sample: 0.3 mg/ml
Injection: 5 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Amines, Herbicide, Organic acid, Pesticide
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Glyphosate, Paraquat