Selectivity Comparison for Amaze PFP Aromatic Phase with Methanol vs. Acetonitrile

Conditions of Experiment
Column: Amaze PFP
Separation Modes: reversed-phase and pi-pi
Column Dimenstions: 4.6x150 mm, 3 μm, 100A
Mobile Phase: ACN/water - 50/50 and MeOH/water - 65/35
Detection: 275 nm
Sample: 0.2 mg/ml
Injection: 2 μL
Flow rate: 1 ml/min
Class of compounds: Aromatic compound
Nature of compounds: Hydrophobic
Compounds: Dimethyl phthalate, Biphenyl, Phenanthrene