HPLC Analysis of Yellow 6 (Sunset Yellow) and Red 40 Dye (Allura Red) in Soft Drinks on Amaze TH Mixed-Mode Column

Application description

Sunset Yellow FCF (Yellow 6) is a synthetic acidic azo dye derived from petroleum. It is used as a coloring agent for food, drug compositions, household products, etc. It is often used in combination with other dyes in candy, snacks, sauces and preserved fruits. This acidic dye was analyzed by HPLC on the Amaze TH mixed-mode HPLC column. The retention time is controlled by the amount of buffer and buffer pH. The maximum absorbance depends on the pH of the mobile phase. This method and the mixed-mode HPLC column can be used for analysis of other acidic dyes in various samples (drinks, food, medications, household cleaners, etc.).

Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC and anion-exchnage
Column Dimenstions: 3.2 x 50 mm, 5 um, 100A
Mobile Phase: MeCN 50% with 50 mM AmFm pH 3
Detection: UV 530 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 0.6 mL/min
Class of compounds: Aromatic acid, Dye
Nature of compounds: Acidic, Hydrophobic, Polar
Compounds: Sunset Yellow, Allura Red