Application description
Aromatic organic acids are used as building blocks in chemistry, additives in various formulations and counter-ions in drug production. In most of the cases, these acids are hydrophilic and acidic in nature with limited retention in reversed-phase chromatography. We have developed a simple, robust and isocratic method to separate mandelic, gallic, phthalic and 4-hydroxybenzoic acids on the Amaze TR HPLC column. All four acids are retained and separated by combination of weak reversed-phase and weak anion-exchange mechanisms. The baseline separation is achieved within 5 minutes. This method can be used for the analysis of hydrophilic and hydrophobic organic and inorganic acids with LC/MS-compatible conditions.
Conditions of Experiment
| Column: |
Amaze TR |
| Separation Modes: |
reversed-phase, anion-exchange |
| Column Dimenstions: |
4.6x50 mm, 3 um, 100A |
| Mobile Phase: |
20% ACN with 20 mM AmFm pH 3 |
| Detection: |
UV 255 nm |
| Sample: |
0.3 mg/ml |
| Injection: |
3 ul |
| Flow rate: |
1 ml/min |
Analytes
| Class of compounds: |
Aromatic acid, Isomer, Organic acid, Preservative |
| Nature of compounds: |
Acidic, Hydrophilic, Hydrophobic, Polar |
| Compounds: |
Mandelic acid, Gallic acid, Phthalic acid, 4-Hydroxybenzoic acid |
