HPLC Analysis of 6 Aromatic Acids and Alcohols on Amaze Aromatic Stationary Phases

Application description

Comparison run on Amaze PFP, Amaze AR, Amaze SPF, and Heritage C20 columns.

Conditions of Experiment
Column: Amaze SPF
Separation Modes: reversed-phase, pi-pi
Column Dimenstions: 4.6x150 mm, 5 μm, 100A
Mobile Phase: 35% ACN with 20 mM AmAc pH 4.5
Detection: UV 235 nm
Sample: 1 mg/ml
Injection: 3 μL
Flow rate: 1 ml/min
Class of compounds: Amines, Aromatic acid, Aromatic base, Aromatic compound, Organic acid, Organic compound, Preservative
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Neutral, Polar
Compounds: 4-Aminophenol, Mandelic acid, 4-Hydroxybenzoic acid, Phenol, Benzoic acid, Methylparaben