HPLC Analysis Four Amino Acids Used in Supplements on Amaze TH Mixed-Mode Column

Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC/cation-exchnage, anion-exchange
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: ACN/water/ammonium formate pH 3
Detection: ELSD
Sample: 0.4-0.6
Injection: 3 uL
Flow rate: 0.6 ml/min
Class of compounds: Amino acid, Inorganic anion, Supplement
Nature of compounds: Acidic, Hydrophilic, Polar, Zwitterionic
Compounds: Chloride Ion, Taurine, Threonine, Serine, Arginine