Quantitative UV HPLC Analysis of Trifluoracetic Acid in Peptide/Protein on Amaze HA Column

Conditions of Experiment
Column: Amaze HA
Separation Modes: HILIC, anion-exchage
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: 60% ACN with 0.1% sulfuric acid
Detection: 215 nm
Sample: 0.1-2 mg/ml
Injection: 2 uL
Flow rate: 0.6 ,ml/min
Class of compounds: Organic acid
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Trifluoroacetic acid