HPLC Analysis of Uridine-Based Nucleotides on Amaze HA Mixed-Mode Column

HPLC Analysis of Uridine-Based Nucleotides on Amaze HA Mixed-Mode Column
Amaze HA PURCHASE LEARN ABOUT Amaze HA
Conditions of Experiment
Column: Amaze HA
Separation Modes: reversed-phase and anion-exchange
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: ACN/Water/Ammonium formate pH 3
Detection: 275 nm
Sample: 0.2-0.4 mg/ml
Injection: 2 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Aromatic acid, Nucleoside, Nucleotide
Nature of compounds: Acidic, Hydrophilic, Neutral
Compounds: Uridine, Uridine 5-monophosphate, Uridine 5-diphosphate, Uridine 5-triphosphate