Ultra-Fast HPLC Separation of Malonic Acids on Coresep SB Column in Reversed-Phase and Anion-Exchange Modes

Application description

Malonic acids are dicarboxylic acids which are polar and thus poorly retained in reversed-phase. An Ultra-fast method for separation of homologs of malonic acids on core-shell mixed-mode columns was developed. Column and method can be used for fast isolation of hydrophilic acids in reversed-phase and anion-exchange modes. Base-line separation with a perfect shape is achieved for all compounds. No ion-pairing reagent is involved.

Conditions of Experiment
Column: Coresep SB
Separation Modes: reversed-phase and anion-exchange
Column Dimenstions: 3.2 x 100 mm, 2.7 um, 90A
Mobile Phase: ACN with sulfuric acid
Detection: UV 215 nm
Sample: 0.3 mg/ml
Injection: 1 uL
Flow rate: 1 ml/min
Class of compounds: Organic acid
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Malonic acid, Methylmalonic acid, Dimethylmalonic acid