HPLC Separation of Isomers of Ascorbic Acid in Reversed-Phase and Anion-Exchange Modes on Amaze HA Column

Conditions of Experiment
Column: Amaze HA
Separation Modes: reversed-phase and anion-exchange
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: 2% ACN with 5 mM Ammonium phosphate pH 4
Detection: 280 nm
Sample: 0.2 mg/ml
Injection: 2 uL
Flow rate: 0.6 ml/min
Class of compounds: Aromatic acid, Organic acid, Supplement, Vitamin
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Isoascorbic acid, Ascorbic acid