HPLC Separation of Isomers of Ascorbic Acid in Reversed-Phase and Anion-Exchange Modes on Amaze HA Column

HPLC-Separation-of-Isomers-of-Ascorbic-Acid-in-Reversed-Phase-and-Anion-Exchange-Modes-on-Amaze-HA-Column
Amaze HA PURCHASE LEARN ABOUT Amaze HA
Conditions of Experiment
Column: Amaze HA
Separation Modes: reversed-phase and anion-exchange
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: 2% ACN with 5 mM Ammonium phosphate pH 4
Detection: 280 nm
Sample: 0.2 mg/ml
Injection: 2 uL
Flow rate: 0.6 ml/min
Analytes
Class of compounds: Aromatic acid, Organic acid, Supplement, Vitamin
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Isoascorbic acid, Ascorbic acid