HPLC Separation of Tricine and Tromethamine on Amaze SC Mixed-Mode Column

Conditions of Experiment
Column: Amaze SC
Separation Modes: reversed-phase cation-exchange
Column Dimenstions: 3.0x100 mm, 5 μm, 100A
Mobile Phase: 5% ACN with 0.1% TFA
Detection: Corona CAD
Sample: 0.5 mg/ml
Injection: 2 μL
Flow rate: 0.6 ml/min
Class of compounds: Amines, Amino acid, Biological buffer
Nature of compounds: Basic, Hydrophilic, Polar
Compounds: Tromethamine, Tricine