Quantitative UV HPLC Analysis of Vitamin C in Juices on Amaze HA Column

Conditions of Experiment
Column: Amaze HA
Separation Modes: HILIC, anion-exchange
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: 60% ACN with 10 mM AmAc pH 4
Detection: 275 nm
Sample: 0.02-0.5 mg/ml
Injection: 2 uL
Flow rate: 0.6 ml/min
Class of compounds: Aromatic acid, Supplement, Vitamin
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Ascorbic acid