Quantitative UV HPLC Analysis of Vitamin C in Juices on Amaze HA Column
Conditions of Experiment
Column: | Amaze HA |
Separation Modes: | HILIC, anion-exchange |
Column Dimenstions: | 3.0x100 mm, 3 um, 100A |
Mobile Phase: | 60% ACN with 10 mM AmAc pH 4 |
Detection: | 275 nm |
Sample: | 0.02-0.5 mg/ml |
Injection: | 2 uL |
Flow rate: | 0.6 ml/min |
Analytes
Class of compounds: | Aromatic acid, Supplement, Vitamin |
Nature of compounds: | Acidic, Hydrophilic, Polar |
Compounds: | Ascorbic acid |