HPLC Analysis of Trifluoroacetic Acid (TFA) on Amaze TH Mixed-Mode Column

Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC, anion-exchange
Column Dimenstions: 4.6 x 100 mm, 5 um, 100A
Mobile Phase: 70% ACN with 0.2% phosphorci acid
Detection: UV 205 nm
Sample: 0.2 mg/ml
Injection: 2 uL
Flow rate: 1 ml/min
Class of compounds: Organic acid
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Trifluoroacetic acid