HPLC Analysis of DMF and N-Nitrosodimethylamine in Metformin Drug on Heritage MC Mixed-Mode Column

Conditions of Experiment
Column: Heritage MC
Separation Modes: reversed-phase, cation-exchnge
Column Dimenstions: 3.0x100 mm, 3 μm, 100A
Mobile Phase: ACN/Water/Formic acid gradient
Detection: UV 230 nm
Sample: 1 mg/ml
Injection: 2μL
Flow rate: 0.6 ml/min
Class of compounds: Drug, Organic compound, Organic solvent
Nature of compounds: Basic, Hydrophilic, Neutral, Polar
Compounds: N-Nitrosodimethylamine, Dimethylformamide, Metformin