Application description
Amino acids serve as building blocks, drugs and nutrients. The usual approach for analysis of amino acids include reversed-phase chromatography with an ion-pairing reagent, derivatization, and hydrophilic interaction chromatography (HILIC). Three amino acids (phenylalanine, tyrosine, and DOPA) were retained and separated in underivatized form in HILIC cation-exchange mode of core-shell mixed-mode HPLC column. This method is fast and reliable and provides base-line separation for all amino acids in the application. Method can be adopted for analysis of other amino acids.
Conditions of Experiment
| Column: |
Coresep S |
| Separation Modes: |
HILIC and cation-exchange |
| Column Dimenstions: |
3.2 x 100 mm, 2.7 um, 90A |
| Mobile Phase: |
85 % ACN with 15 mM AmAc pH 5 |
| Detection: |
UV 270, ELSD |
| Sample: |
0.3 mg/ml |
| Injection: |
3 uL |
| Flow rate: |
0.5-2 ml/min |
Analytes
| Class of compounds: |
Amino acid, Catecholamine, Drug, Neurotransmitter, Supplement |
| Nature of compounds: |
Basic, Hydrophilic, Polar, Zwitterionic |
| Compounds: |
Phenylalanine, Tyrosine, DOPA |
