HPLC Analysis of Underivatized Amino Acids on Coresep S Column in HILIC and Cation-Exchange Modes

Application description

Amino acids serve as building blocks, drugs and nutrients. The usual approach for analysis of amino acids include reversed-phase chromatography with an ion-pairing reagent, derivatization, and hydrophilic interaction chromatography (HILIC). Three amino acids (phenylalanine, tyrosine, and DOPA) were retained and separated in underivatized form in HILIC cation-exchange mode of core-shell mixed-mode HPLC column. This method is fast and reliable and provides base-line separation for all amino acids in the application. Method can be adopted for analysis of other amino acids.

Conditions of Experiment
Column: Coresep S
Separation Modes: HILIC and cation-exchange
Column Dimenstions: 3.2 x 100 mm, 2.7 um, 90A
Mobile Phase: 85 % ACN with 15 mM AmAc pH 5
Detection: UV 270, ELSD
Sample: 0.3 mg/ml
Injection: 3 uL
Flow rate: 0.5-2 ml/min
Class of compounds: Amino acid, Catecholamine, Drug, Neurotransmitter, Supplement
Nature of compounds: Basic, Hydrophilic, Polar, Zwitterionic
Compounds: Phenylalanine, Tyrosine, DOPA