HPLC Analysis of Dextromethorphan and Counter-Ion on Coresep SB Column in Reversed-Phase Mixed Mode

Application description

Dextromethorphan and bromide counter-ion were analyzed by mixed-mode HPLC on core-shell reversed-phase anion exchange column. Dextromethorphan is retained by strong hydrophobic interaction and the bromide ion is retained by an anion-exchange interaction. Order of elution for basic drug and it’s acidic counter-ion can be changed by modifying the amount of ACN, buffer pH, and buffer concentration. The Coresep SB column can be used for analysis of hydrophobic basic drugs and acidic counter-ions in one run without a ion-pairing reagent. Compounds can be monitored by all common detection techniques.

Conditions of Experiment
Column: Coresep SB
Separation Modes: reversed-phase, anion-exchange and cation-exclusion
Column Dimenstions: 3.2 x 100 mm, 2.7 um, 90A
Mobile Phase: 20% ACN with 0.1% or 0.2% TFA
Detection: UV 255 nm
Sample: 0.3 mg/ml
Injection: 3 uL
Flow rate: 0.6 ml/min
Class of compounds: Amines, Aromatic base, Drug
Nature of compounds: Basic, Hydrophobic
Compounds: Dextromethorphan