HPLC Separation of Isomers of Ascorbic Acid in Reversed-Phase and Anion-Exchange Modes on Coresep SB Column

Conditions of Experiment
Column: Coresep SB
Separation Modes: reversed-phase and anion-exchange
Column Dimenstions: 4.6x150 mm, 2.7 um , 100A
Mobile Phase: 20% ACN with 50 mM Ammonium phosphate pH 4
Detection: 280 nm
Sample: 0.2 mg/ml
Injection: 2 uL
Flow rate: 1 ml/min
Class of compounds: Aromatic acid, Organic acid, Supplement, Vitamin
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Ascorbic acid, Isoascorbic acid