HPLC Analysis of Six Aldehydes on Amaze C18 and Eclipse XDB-C18 Columns

Conditions of Experiment
Column: Amaze C18
Separation Modes: reversed-phase
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: 15% ACN with 50 mM ammonium phosphate and 0.1% phosphoric acid
Detection: 225 nm
Sample: 0.3-0.5 mg/ml
Injection: 1 uL
Flow rate: 1 ml/min
Class of compounds: Aldehyde, Aromatic compound
Nature of compounds: Hydrophobic
Compounds: Furfural, 5-Methylfurfural, Vanillin, Syringic aldehyde, Ethylvanillin, Coniferyl aldehyde