HPLC UV Analysis of Polyethyleneimine on Heritage MA Column in Ion-Exclusion and Size-Exclusion Modes

Conditions of Experiment
Column: Heritage MA
Separation Modes: cation-exclusion, size-exclusion
Column Dimenstions: 4.6x250 mm, 3 um, 100A
Mobile Phase: 30% ACN with 30 mM AmAc pH 4
Detection: UV 275 nm
Sample: 0.3 mg/ml
Injection: 5 uL
Flow rate: 0.25 ml/min
Class of compounds: Amines, Polymer
Nature of compounds: Basic, Hydrophilic, Polar
Compounds: Polyethylenimine