HPLC Analysis of Glyphosate and Ethephon on Amaze HA Mixed-Mode Column with LC/MS Compatible Conditions

Conditions of Experiment
Column: Amaze HA
Separation Modes: HILIC, anion-exchange
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: 60% ACN with 20 mM AmFm pH 3
Detection: ELSD
Sample: 0.5 mg/ml
Injection: 2 uL
Flow rate: 0.6 ml/min
Class of compounds: Herbicide, Organic acid, Pesticide
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Glyphosate, Ethephon