HPLC Separation of Dihydrostreptomycin and Streptomycin in HILIC, Cation-Exchange Modes on Amaze TCH Column

Conditions of Experiment
Column: Amaze TCH
Separation Modes: HILIC and cation-exchange
Column Dimenstions: 3.0x100 mm, 3 um, 100A
Mobile Phase: 75% ACN with 50 mM AmFm pH 2.7
Detection: ELSD
Sample: 0.3 mg/ml
Injection: 2 uL
Flow rate: 0.6 ml/min
Class of compounds: Amines, Aminoglycoside, Antibiotic, Drug
Nature of compounds: Basic, Hydrophilic
Compounds: Dihydrostreptomycin, Streptomycin