HPLC Separation of Leucine and Isoleucine in HILIC, Cation-Exchange Modes on Amaze TCH Column

Conditions of Experiment
Column: Amaze TCH
Separation Modes: HILIC, cation-exchange, anion-exchange
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: 75% ACN with 15 mM AmFm pH 2.7
Detection: ELSD
Sample: 0.5 mg/ml
Injection: 2 uL
Flow rate: 1 ml/min
Class of compounds: Amino acid, Supplement
Nature of compounds: Hydrophilic, Polar, Zwitterionic
Compounds: Leucine, Isoleucine