HPLC Analysis of Drug Loratadine and Related Impurities on Heritage MC Mixed-Mode Column
Application description
Loratadine is a drug which is used to treat allergies. It is available in combination with pseudoephedrine in different forms including tablets, oral suspensions, and syrop. Loratadine and related impurities were separated on Heritage MC mixed-mode column by combination of reversed-phase and cation-exchange mechanisms. Retention time is controlled by amount of ACN, buffer concentration, and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of loratadine in various samples. Various mobile phases can be used with Heritage MC column to accommodate different detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection.
Conditions of Experiment
Column: | Heritage MC |
Separation Modes: | reversed-phase and cation-exchange |
Column Dimenstions: | 4.6 x 150 mm |
Mobile Phase: | 60% ACN with 60 mM AmAc pH 5 |
Detection: | UV 255 nm |
Sample: | 0.3 mg/ml |
Injection: | 3 uL |
Flow rate: | 1 mL/min |
Analytes
Class of compounds: | Aromatic acid, Aromatic base, Drug, Pyridine |
Nature of compounds: | Basic, Hydrophilic, Hydrophobic |
Compounds: | Loratadine, Related Impurities |