HPLC Analysis of Drug Loratadine and Related Impurities on Heritage MC Mixed-Mode Column

HPLC Analysis of Drug Loratadine and Related Impurities on Heritage MC Mixed-Mode Column chromatogram
Conditions of Experiment
Column:
Heritage MC
Separation Modes:
reversed-phase and cation-exchange
Column Dimenstions: 4.6 x 150 mm
Mobile Phase: 60% ACN with 60 mM AmAc pH 5
Detection: UV 255 nm
Sample: 0.3 mg/ml
Injection: 3 uL
Flow rate: 1 mL/min
Analytes
1. Loratadine
2. Related Impurities

Class of compounds: Aromatic acid, Aromatic base, Drug, Pyridine
Nature of compounds: Basic, Hydrophilic, Hydrophobic

Application description

Loratadine is a drug which is used to treat allergies. It is available in combination with pseudoephedrine in different forms including tablets, oral suspensions, and syrop. Loratadine and related impurities were separated on Heritage MC mixed-mode column by combination of reversed-phase and cation-exchange mechanisms. Retention time is controlled by amount of ACN, buffer concentration, and buffer pH. Method is fast, robust, reproducible, and can be used for analysis of loratadine in various samples. Various mobile phases can be used with Heritage MC column to accommodate different detection techniques. UV transparent buffers can be replaced with volatile buffers for MS, ELSD or CAD detection.

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