HPLC Analysis of 6 Aromatic Acidic, Zwitterionic and Neutral Compounds on Amaze Aromatic Stationary Phases

Application description

Comparison run on Amaze PFP, Amaze AR, Amaze SPF, and Heritage C20 columns.

Conditions of Experiment
Column: Amaze AR
Separation Modes: reversed-phase, pi-pi
Column Dimenstions: 4.6x150 mm, 5 μm, 100A
Mobile Phase: 28% ACN with 20 mM AmAc pH 4.5
Detection: UV 255 nm
Sample: 1 mg/ml
Injection: 3 μL
Flow rate: 1 ml/min
Class of compounds: Amino acid, Aromatic acid, Aromatic compound, Drug, Isomer, Organic acid, Preservative, Stimulant, Xanthine
Nature of compounds: Acidic, Hydrophilic, Hydrophobic, Neutral, Polar, Zwitterionic
Compounds: Caffeine, 4-Aminobenzoic acid, Aspirin, 4-Hydroxybenzoic acid, Sorbic acid, Benzoic acid