HPLC Analysis of Six Organic Acids on Heritage MA Mixed-Mode Column

Conditions of Experiment
Column: Heritage MA
Separation Modes: reveresed-phase, anion-exchange
Column Dimenstions: 4.6 x 150 mm, 5 um, 100A
Mobile Phase: 35% ACN with 50 mM AmFm pH 3
Detection: UV 255 nm
Sample: 0.2 mg/ml
Injection: 5 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Aromatic acid, Organic acid, Preservative
Nature of compounds: Acidic, Hydrophilic, Hydrophobic, Polar
Compounds: Gallic acid, 4-Hydroxybenzoic acid, Caffeic acid, Mandelic acid, Maleic acid, Atrolactic acid