HPLC Analysis of 10 Water-Soluble Vitamins in HILIC, Cation- and Anion-Exchange Modes on Amaze TH Tri-Modal Column

Conditions of Experiment
Column: Amaze TH
Separation Modes: reversed-phase, cation-exchange, anion-exchange
Column Dimenstions: 4.6x150 mm, 3 μm, 100A
Mobile Phase: ACN from 87% to 40%, AmFM pH 3 from 10 mM 60 mM in 10 min, 7 min hold
Detection: UV 275 nm, ELSD
Sample: 1 ml/min
Injection: 3 μL
Flow rate: 1 ml/min
Class of compounds: Amines, Aromatic acid, Aromatic base, Organic acid, Supplement, Vitamin
Nature of compounds: Acidic, Basic, Hydrophilic, Hydrophobic, Polar
Compounds: Nicotinamide, Biotin, Panthothenic acid, Riboflavin, Pyridoxine, Ascorbic acid, Niacin, Folic acid, Cyanocobalamin, Thiamine