HPLC Analysis of Five Acidic Drugs on Heritage MA Mixed-Mode Column

Conditions of Experiment
Column: Heritage MA
Separation Modes: reversed-phase and anion-exchange
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: 35% ACN with 0.15% sulfuric acid
Detection: 255 nm
Sample: 0.2-0.4 mg/ml
Injection: 2 uL
Flow rate: 1 ml/min
Class of compounds: Aromatic acid, Drug, Organic acid
Nature of compounds: Acidic, Hydrophilic, Hydrophobic, Neutral, Polar
Compounds: Aspirin, Salicylic acid, Ketoprofen, Naproxen, Ibuprofen