HPLC Analysis of Ascorbic, Citric and Ethylenediaminetetraacetic acids on Heritage MA Mixed-Mode Column

Conditions of Experiment
Column: Heritage MA
Separation Modes: reversed-phase and anion-exchange
Column Dimenstions: 4.6x50 mm, 3 μm, 100A
Mobile Phase: ACN gradient from 2% to 40%, phosphoric acid gradient from 0.03% to 0.4% in 5 min, 2 min hold
Detection: UV 220 nm
Sample: 1 mg/ml
Injection: 2 μL
Flow rate: 1 ml/min
Class of compounds: Aromatic acid, Organic acid, Preservative, Supplement, Vitamin
Nature of compounds: Acidic, Hydrophilic, Polar, Zwitterionic
Compounds: Ascorbic acid, Citric acid, Ethylenediaminetetraacetic acid