HPLC Separation of Sodium and Four Inorganic Anions on Amaze TH Column

Conditions of Experiment
Column: Amaze TH
Separation Modes: HILIC cation-exchange, anion-exchange
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: 40% ACN with gradient of AmFm pH 3 from 30 mM
to 120 mM in 7.5 min, hold 8 min
Detection: ELSD
Sample: 0.5-1 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Inorganic acid, Inorganic anion, Inorganic cation, Organic acid
Nature of compounds: Acidic, Basic, Hydrophilic, Polar
Compounds: Phosphate Ion, Chloride Ion, Sodium ion, Iodide ion, Sulfate ion