HPLC Separation of Sodium and Four Inorganic Anions on Amaze TH Column
Conditions of Experiment
Column: | Amaze TH |
Separation Modes: | HILIC cation-exchange, anion-exchange |
Column Dimenstions: | 4.6x150 mm, 3 um, 100A |
Mobile Phase: | 40% ACN with gradient of AmFm pH 3 from 30 mM to 120 mM in 7.5 min, hold 8 min |
Detection: | ELSD |
Sample: | 0.5-1 mg/ml |
Injection: | 3 uL |
Flow rate: | 1 ml/min |
Analytes
Class of compounds: | Inorganic acid, Inorganic anion, Inorganic cation, Organic acid |
Nature of compounds: | Acidic, Basic, Hydrophilic, Polar |
Compounds: | Phosphate Ion, Chloride Ion, Sodium ion, Iodide ion, Sulfate ion |