Nicotinamide
HPLC Analysis of Substituted Pyridines on Amaze SC Mixed-Mode Column
		
		
			
			
				
		
	
					Conditions of Experiment
					
						
				
				| Column: | Amaze SC | 
| Separation Modes: | reversed-phase, cation-exchange | 
| Column Dimenstions: | 4.6x150 mm, 5 um, 100A | 
| Mobile Phase: | 5% ACN with 0.1% phosphoric acid | 
| Detection: | UV 255 nm | 
| Sample: | 0.2-0.3 mg/ml | 
| Injection: | 2 uL | 
| Flow rate: | 1 ml/min | 
					Analytes
					
						
				
			| Class of compounds: | Amino acid, Aromatic base, Aromatic compound, Isomer, Pyridine, Vitamin | 
| Nature of compounds: | Basic, Hydrophilic, Polar, Zwitterionic | 
| Compounds: | 2-Pyridinecarboxylic acid, 3-Pyridinecarboxylic acid, 4-Pyridinecarboxylic acid, Nicotinamide, Pyridoxal, Pyridoxine | 
HPLC Analysis of 10 Water-Soluble Vitamins in HILIC, Cation- and Anion-Exchange Modes on Amaze TH Tri-Modal Column
		
		
			
			
				
		
	
					Conditions of Experiment
					
						
				
				| Column: | Amaze TH | 
| Separation Modes: | reversed-phase, cation-exchange, anion-exchange | 
| Column Dimenstions: | 4.6x150 mm, 3 μm, 100A | 
| Mobile Phase: | ACN from 87% to 40%, AmFM pH 3 from 10 mM 60 mM in 10 min, 7 min hold | 
| Detection: | UV 275 nm, ELSD | 
| Sample: | 1 ml/min | 
| Injection: | 3 μL | 
| Flow rate: | 1 ml/min | 
					Analytes
					
						
				
			| Class of compounds: | Amines, Aromatic acid, Aromatic base, Organic acid, Supplement, Vitamin | 
| Nature of compounds: | Acidic, Basic, Hydrophilic, Hydrophobic, Polar | 
| Compounds: | Nicotinamide, Biotin, Pantothenic acid, Riboflavin, Pyridoxine, Ascorbic acid, Niacin, Folic acid, Cyanocobalamin, Thiamine | 
