Direct HPLC Analysis Etidronic Acid in Urine on Amaze HA Mixed-Mode Column

Conditions of Experiment
Column: Amaze HA
Separation Modes: anion-exchange
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: 75% ACN with 30 mM AmFm pH 3
Detection: Corona CAD, 235 nm
Sample: 0.1 mg/ml
Injection: 3 uL
Flow rate: 1 ml/min
Class of compounds: Drug, Organic acid
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Etidronic acid