Direct HPLC Analysis of Acidic Drug Aspirin in Human Blood on Heritage MA Mixed-Mode Column

Conditions of Experiment
Column: Heritage MA
Separation Modes: anion-exchange
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: 75% ACN with 15 mM AmAc pH 4
Detection: 235 nm
Sample: 0.1 mg/ml
Injection: 1 uL
Flow rate: 1 ml/min
Class of compounds: Aromatic acid, Drug
Nature of compounds: Hydrophobic, Neutral, Polar
Compounds: Aspirin