Direct HPLC Analysis of Salicylic Acid in Human Blood on Heritage MA Mixed-Mode Column

Direct HPLC Analysis of Salicylic Acid in Human Blood on Heritage MA Mixed-Mode Column chromatogram0
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Conditions of Experiment
Column: Heritage MA
Separation Modes: reversed-phase and anion-exchange
Column Dimenstions: 4.6x150 mm, 3 um, 100A
Mobile Phase: 75% ACN with 15 mM AmAc pH 4
Detection: 235 nm
Sample: 0.1 mg/ml
Injection: 1 uL
Flow rate: 1 ml/min
Analytes
Class of compounds: Aromatic acid, Drug, Organic acid
Nature of compounds: Acidic, Hydrophilic, Polar
Compounds: Salicylic acid